Stylet probing activities of transgenic and wild type Peregrinus maidis planthoppers

Sunday, November 13, 2022

3:30 PM – 3:42 PM PT

Location: Vancouver Convention Centre, Meeting Room 202

Presenting Author(s)

AW

Astri C. Wayadande

Professor
Oklahoma State University
Stillwater, Oklahoma

Co-Author(s)

WK

Will Klobasa

Oklahoma State University
Stillwater, Oklahoma
CX

Cesar Xavier

North Carolina State University
Raleigh, North Carolina
ML

Marcé Lorenzen

North Carolina State University
Raleigh, North Carolina
DR

Dorith Rotenberg

North Carolina State University
Raleigh, North Carolina
Anna E. Whitfield

North Carolina State University
Raleigh, North Carolina

The probing activities of Cas9 modified male Peregrinus maidis adults were measured using electropenetrography (EPG) while feeding on young 3-4 leaf maize plants and the resulting electropenetrographs were annotated, quantified, and compared to those produced by wild type males. Cas 9 males produced waveforms that were indistinguishable from those produced by wild type males. Each cohort (wild type and Cas9) probed in two phases: pathway phase and ingestion phase. Pathway phase consisted of stylet penetration into plant tissues, then movement of stylets and secretion of the salivary sheath that anchors the stylets until vascular tissues were reached. Ingestion phase consisted of both phloem probing and xylem ingestion. Probing frequencies and durations of specific activities, such as pathway, phloem ingestion, and xylem ingestion were very similar between the two cohorts of P. maidis.