New Information on RNAi Mechanisms and Technological Advances toward Practical Applications

Organizer(s)

• KN

  Kenneth Narva

  Head of Entomology, Research Fellow
  Department of Entomology
  GreenLight Biosciences
  Research Triangle Park, North Carolina
• 

  Ana Velez

  Assistant Professor Department of Entomology
  Department of Entomology
  University of Nebraska
  Lincoln, Nebraska
• MD

  Molly Darlington

  Graduate Research Assistant
  Department of Entomology
  University of Nebraska
  Lincoln, Nebraska
• 

  Swati Mishra

  Graduate research assistant
  Department of Entomology and Plant Pathology
  University of Tennessee
  Knoxville, Tennessee

Presentations:

• 8:00 AM – 8:05 AM PT

  Introductory remarks
  

  Presenting Author: Kenneth Narva – GreenLight Biosciences
• 8:05 AM – 8:20 AM PT

  Mechanisms of double-stranded RNA uptake in the western corn rootworm
  

  Presenting Author: Molly Darlington – University of Nebraska

  Co-Author: Ana M. Velez – University of Nebraska
• 8:20 AM – 8:35 AM PT

  Role of uptake and detoxification in CPB (Leptinotarsa decemlineata) resistance to dsRNA
  

  Presenting Author: Swati Mishra – University of Tennessee

  Co-Author: Kurt H. Lamour – University of Tennessee

  Co-Author: Scott Emrich – University of Tennessee

  Co-Author: William J. Moar – Bayer Crop Science

  Co-author: Scott Lenaghan – University of Tennessee

  Co-Author: Juan Jurat-Fuentes – University of Tennessee
• 8:35 AM – 8:50 AM PT

  Comparison of dsRNA, siRNA and shRNA uptake and efficiency in insect cells
  

  Presenting Author: Jinmo Koo (he/him/his) – University of Kentucky

  Co-author: Dhandapani Gurusamy – University of Kentucky

  Co-Author: Subba Palli – University of Kentucky
• 8:50 AM – 9:05 AM PT

  Alternative structured dsRNAs and their potential to enhance RNAi in insects
  

  Presenting Author: Steve Whyard – University of Manitoba

  Co-author: Alison Tayler – University of Manitoba

  Co-author: Nazanin Amanat – University of Manitoba

  Co-author: Aditi Sing – University of Manitoba
• 9:05 AM – 9:20 AM PT

  Opportunities offered by piRNA mediated gene silencing in pest control
  

  Presenting Author: Alex Flynt – University of Southern Mississippi

  Co-author: Mosharrof Mondal – RNAissance Ag, LLC

  Co-author: Beatriz Zanacanela – University of Southern Mississippi

  Co-author: Abel Gurung – University of Southern Mississippi

  Co-Author: Judith K. Brown – University of Arizona
• 9:20 AM – 9:35 AM PT

  Viruses as efficient vehicles to improve RNAi efficiency in insects
  

  Presenting Author: Clauvis Nji Tizi Taning – Ghent University
• 9:35 AM – 9:50 AM PT

  Break
• 9:50 AM – 10:05 AM PT

  Potential of RNAi as a tool for the control of the two-spotted spider mite, Tetranychus urticae
  

  Presenting Author: Vojislava Grbic – University of Western Ontario

  Co-author: Zoran Culo – University of Western Ontario

  Co-author: Joseane Moreira do Nascimento – University of Western Ontario

  Co-author: Nicolas Bensoussan – University of Western Ontario

  Co-author: Sameer Dixit – University of Western Ontario

  Co-author: Vladimir Zhurov – University of Western Ontario
• 10:05 AM – 10:20 AM PT

  Low-cost dsRNA manufacturing by microbial fermentation: The key to accelerating and broadening RNAi-based biopesticide development and deployment
  

  Presenting Author: Candace Seeve – TechAccel, LLC

  Co-author: Anil Kumar – RNAissance Ag, LCC

  Co-author: Camila Oliveira-Hofman – RNAissance Ag, LLC

  Co-author: Mosharrof Mondal – RNAissance Ag, LLC

  Co-author: Fred Moshir – RNAissance Ag, LLC

  Co-author: Jacob Shoemake – RNAissance Ag, LLC

  Co-author: Tammy Zanker – RNAissance Ag, LLC

  Co-author: Dylan A. Gladson – RNAissance Ag, LLC

  Co-author: Bala P. Venkata – Donald Danforth Plant Science Center

  Co-author: Brad Fabbri – TechAccel, LLC
• 10:20 AM – 10:35 AM PT

  Effects of SmartStax and SmartStax PRO maize on western corn rootworm larval feeding injury and adult life history traits
  

  Presenting Author: Jordan D. Reinders – University of Nebraska

  Co-Author: William J. Moar – Bayer Crop Science

  Co-Author: Graham P. Head – Bayer Crop Science

  Co-author: Safeer Hassan – Bayer Crop Science

  Co-Author: Lance J. Meinke – University of Nebraska
• 10:35 AM – 10:50 AM PT

  Colorado potato beetle control with RNAi: Insights from laboratory and field experiments using ledprona
  

  Presenting Author: Andrei Alyokhin – University of Maine
• 10:50 AM – 11:05 AM PT

  Calantha^TM: A new tool for controlling Colorado potato beetle, Leptinotarsa decemlineata, in potato
  

  Presenting Author: Brian S. Manley – GreenLight Biosciences

  Co-Author: Ethann Barnes – GreenLight Biosciences

  Co-Author: Thais Rodrigues – GreenLight Biosciences

  Co-Author: Russell Groves – University of Wisconsin

  Co-Author: Andrei Alyokhin – University of Maine

  Co-Author: Brian Nault – Cornell University

  Co-Author: Timothy Waters – Washington State University

  Co-Author: Silvia I. Rondon – Oregon State University

  Co-Author: Thomas P. Kuhar – Virginia Polytechnic Institute and State University

  Co-Author: Erik J. Wenninger – University of Idaho
• 11:05 AM – 11:20 AM PT

  Panel discussion
• 11:20 AM – 11:25 AM PT

  Closing remarks
  

  Presenting Author: Kenneth Narva – GreenLight Biosciences