PhD Candidate Universidad de Guadalajara Zapopan, Jalisco, Mexico
Abstract Text: Ankylosing Spondylitis (AS) is an inflammatory autoimmune disease of unknown etiology. In Mexico the prevalence is estimated at 0.09% and the overwhelming risk factor for developing AS is the presence of HLA-B27. However, others genes could also be involved in AS development. DNAM-1, an activating receptor for CD8+ T lymphocytes and NK cells, participates in the regulation of cytotoxicity and the release of cytokines. Several reports have associated single nucleotide polymorphisms (SNPs) rs.763361 C>T and rs.727088 G>A in the DNAM-1 gene with different autoimmune pathologies. These SNPs are associated with decreased expression of both mRNA and protein, and decreased cell activation; however, the role of DNAM-1 in AS is currently unknown. For this reason, the aim of this work was to analyze the association of these SNPs with the BASDAI index, CRP levels and AS. We enrolled 35 patients with AS and 75 healthy subjects; genotyping of the polymorphisms rs.763361 C>T and rs.727088 G>A was performed using end-point PCR. Our results showed that rs.763361 C> T and rs.727088 G> A are not associated with the susceptibility to develop AS (p>0.005). However, the haploytpe analysis showed the T+A haplotype as a protective factor (OR = 0.35, 95%CI = 0.15-0.78, p= 0.012) for AS development. Also, the TT genotype of rs.763361 C> T was associated with an increased BASDAI index (p < 0.005). In conclusion, DNAM-1 could be involved in the physiopathology of AS.
