Th84 - Leveraging the OASIS Resource to Query LEAP GWAS Results in an Unbiased or Targeted Fashion

Intro: Genome wide association studies of the Learning Early about Peanut (LEAP) study participants has resulted in multiple publications and a vast amount of data remains to be interrogated.  Given the quantity of data, we determined that there was a need to make this information publicly available to query in a way that is “scientist friendly”.

Methods: A new online platform, the Omics Analysis, Search, and Information System (OASIS), was developed to provide a user-friendly platform for interrogating LEAP GWAS data.  OASIS integrates this data with functional annotations from GTEx and a wealth of bioinformatics resources.  On demand analyses including visualization of linkage disequilibrium and Manhattan plots can easily be generated.

Results: OASIS can be queried in an unbiased fashion or for genes or single nucleotide polymorphisms (SNP) of interest.  To illustrate targeted use of the database, a query for CMA1, a gene previously associated with eczema and IgE levels in eczema, was performed.  The peak association for CMA1 was SCORAD at 12 months study duration (OR=15 and p< 0.001).  CMA1 was also associated with peanut-specific IgG4 at multiple timepoints suggesting that CMA1 may be associated with development of blocking antibodies.  CMA1 appeared to have a relationship to peanut allergy status at 60 months in those avoiding peanut (OR 9.2, p=0.099).  CMA1 may not only be associated with total IgE levels in patients with eczema, but also in clinical food allergy status.

Conclusions: OASIS is a “scientist friendly” web-based system for mining genetic associations in the LEAP GWAS dataset.