LB 2-8 - Identification of seasonal spatial distribution of specific species in urban reservoirs using eDNA technology

Background/Question/Methods:

The lake located within the city park is affected by the surrounding environment and the ecosystem is constantly disturbed. In order to conserve biodiversity, it is necessary to identify the distribution of species in a timely manner, but it is difficult to grasp the distribution of many species due to the different habitat conditions of the existing monitoring methods. Therefore, the use of environmental DNA technology useful for species detection through information remaining in environmental samples is increasing. The purpose of study is to propose monitoring and management measures using eDNA to identify the appearance and seasonal distribution of specific in urban lakes. A total of 21 points were selected as the target sites, including Woncheon lake, Sindae lake and Woncheon stream in Gwanggyo Lake Park, Yeongtong-gu, Suwon-si, Gyeonggi-do. Using a disposable syringe, a volume of about 200 to 500 ml was filtered on 0.45 μm cartridge filter paper. Filtered samples were subjected to Next Generation Sequencing using the primers presented in Ushio et al (2017).

Results/Conclusions:

As a result of this study, there was a difference in the distribution width and value for each season according to tolerance characteristics. In particular, the endogenous species in Woncheon Lake was stable in May, October. The dominance of endogenous species in Wonchoen Lake in May was the lowest at 0.007, and the species diversity and evenness were high at 0.064 and 0.024, confirming that they were in a stable community condition compared to other places. Also, compared to the month of May, the intermediate species showed a positive correlation with salinity compared to the resistant species, and a difference was found in October compared to May in three places. This study is meaningful in identifying the distribution by species and seasons by considering the habitat environment of the urban water purification ecosystem through eDNA, and furthermore, it can suggest management plans for exotic species.