259 - New approaches for global cell signaling

Session Chair(s)

• Vincent Tagliabracci

  University of Texas, Southwestern

In this session, we will focus on atypical signaling mechanisms, from the discovery of new catalysis within the kinome superfamily and noncanonical ubiquitination to the role of metals such as copper in signaling. We also discuss the emergence of pseudoenzymes: These allosteric signaling scaffolds are defined by their structural and sequence homology to canonical enzymes such as kinases and phosphatases, but they lack catalytic activity and remain relatively unexplored biologically and as potential drug targets. We also will discuss how improvements in phosphoproteomics, genetic screens, and affinity and proximity proteomics permit us to globally assess specific aspects of signal transduction and shine new lights on poorly characterized enzymes, scaffolds and substrates.

Who should attend: everyone who likes taking the road less traveled and those interested in good detective stories.

Presentations:

• 9:15 AM – 9:45 AM

  Targeted CRISPR screens identify context-specific signaling dependencies in 30 patient-derived GBM stem cell cultures
  

  Presenting Author: Graham MacLeod – University of Toronto
• 9:45 AM – 10:15 AM

  Proximity-dependent sensors for signaling
  

  Presenting Author: Anne-Claude Gingras – Lunenfeld-Tanenbaum Research Institute, University of Toronto
• 10:15 AM – 10:45 AM

  Proteome-scale amino-acid resolution footprinting of protein-binding sites in the intrinsically disordered regions
  

  Presenting Author: Ylva Ivarsson – Uppsala University
• 10:45 AM – 11:15 AM

  Protein sumoylation dynamic signaling unveiled using quantitative proteomics
  

  Presenting Author: Pierre Thibault – Université de Montréal