Senior Research Fellow, Presenting Author
Promega Corp.
Said A. Goueli, Ph.D.
Senior Research Fellow, and Group Leader of Cell Signaling
Promega Corporation
Said Goueli is a Senior Research Fellow at Promega Corporation. He is the founder of the cell signaling group, where his focus is mainly directed at biochemical assays to study cell signaling and, most recently, on cell based technologies to monitor cellular metabolites in normal and abnormal cell growth.
Said has pioneered novel technologies that advanced research in cell signaling. He was one of the first to develop anti phosphospecific antibodies, and holds over 15 issued and several pending patents on kinase assays (radioactive, fluorescent, and luminescent) and related technologies. He has also developed novel technologies in monitoring the activity of protein phosphatases and is currently developing luminescent assays for monitoring the activity of phospholipid phosphatases.
Said has also developed a novel strategy to monitor the modulation of Gs and Gi-Protein Coupled Receptors (GPCR) and Phosphodiesterases. His interest has expanded to newer areas of cell signaling research as evidenced by his development of assays to address the need of research in the epigenome. His most recent development is a universal homogenous bioluminescent assay for all classes of methyltransferases.
Said most recent activities are devoted to monitoring and detection of the activity of small GTPases such as RAS, RHO, RAK, CDC42, etc. which are intimately involved in the development and proliferation of almost all kinds of tumors and play pivotal role in tumor invasiveness and metastasis.
The quality of air, food, and water has recently draw Said attention to develop novel approaches to detect bacteria, yeast, and spores and thus can be used as quality control measures.
Prior to joining Promega, Said was at the University of Minnesota, where he made seminal contributions to the molecular action of androgens on modulating gene expression in the prostate.
Said has published over 100 peer reviewed journals and over 15 chapters, as well as organized many symposia on cell signaling. He also is a Co- editor of a volume on Protein Kinases in the series of “Methods in Molecular Biology”. He has over 16 Patents in the areas of kinases and GPCR.
Email – Said.Goueli@promega.com
Phone – 608-277-2650
Selected Publications:
1. Swiatnicki, ... Said A Goueli, Hicham Zegzouti (2022) Profiling oncogenic KRAS mutant drugs with a cell-based Lumit p-ERK immunoassay. SLAS Discov. Mar 11; S2472-5552(22)12517-7.
2. Alves, ...., and Said A Goueli, Hicham Zegzouti (2021) A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels. Sci Reports. 11(1):18428
3. Engel, .... and Said A. Goueli, and Hicham Zegzouti (2021) Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors. Molecules, 26(20), 6230;
4. Abushaban, A;... SG; Goueli, (2020). Monitoring Biofouling Potential Using ATP-Based Bacterial Growth Potential in SWRO Pre-Treatment of a Full-Scale Plant. Membranes, volume 10: Issue 11, 360
5. Huang, B, Engel, L, Goueli, SA, and Zegzouti, H (2020) A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Nature Communication Biology, 3: 8. Published online 2020 Jan 3. doi: 10.1038/s42003-019-0723-9
6. Goueli, SA and Hsiao, K (2019) Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39.
PLoS One. 2019 Oct 25;14(10)
7. Abushaban,.. S, Goueli, SA, Schippers, JC, and Kennedy, MD (2017) Direct measurement of ATP in seawater and application of ATP to monitor bacterial growth potential in SWRO pre-treatment systems. Desalination and Water Treatment, 99:91-101
8. Alves J, Vidugiris G, Goueli SA, Zegzouti H (2017) Bioluminescent High-Throughput Succinate Detection Method for Monitoring the Activity of JMJC Histone Demethylases and Fe(II)/2-Oxoglutarate-Dependent Dioxygenases. SLAS Discov. 2017
9. Mondal S, Hsiao K, Goueli SA (2017) Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions. Assay Drug Dev Technol...15; 330-341.
10. Hsiao K, .. Goueli S. (2017) A High-Throughput Bioluminescent Assay to Monitor the Deamidation of Asparagine and Isomerization of Aspartate Residues in Therapeutic Proteins and Antibodies. J Pharm Sci. 106:1528-1537.
11. Goueli, SA (2017) Kinases and Drug Discovery: Protein Kinases as a Validated Drug Target (2107) Drug Discovery World. pp53-60
12. Zegzouti, H, Hennek, J, and Goueli, SA (2016) Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity. In “Methods in Molecular Biology: Kinase Screening and Profiling; Methods and Protocols; (Zegzouti and Goueli, eds.) Humana Press”. pp 59-73.
13. Mondal S, Hsiao, K, and Goueli, SA (2016) A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510, 41-51
14. Hsiao, K; Zegzouti, H; and Goueli, SA (2016) Methyltransferase-Glo: A universal, bioluminescent and homogenous assay for monitoring all classes of methyltransferases. J. Epigenomics, Vol. 8, 321-339.
15. Mondal S, Hsiao K, Goueli SA (2015) A Homogenous Bioluminescent System for Measuring GTPase, GTPase Activating Protein, and Guanine Nucleotide Exchange Factor Activities. Assay Drug Dev Technol. 8:444-455
16. Goueli, SA, Zegzouti, H, and Hsiao K (2014) AMP Glo, Luminescent assay for diverse Biochemical Reactions. GEN, August issue
17. Zegzouti, H;...and Goueli, SA (2009) ADP-GLO: A Bioluminescent and homogenous ADP monitoring assay for kinases. Assay Drug Dev. Technol. 7: 560-572.
18. Vidugiriene, J; Zegzouti, H; and Goueli, SA (2009) Evaluating the utility of a bioluminescent ADP-detecting assay for lipid kinases. Assay Drug Dev. Technol. 7: 585-597
19. El Awady, .. and Goueli, SA (2009) Positional effect of mutations in 5’UTR of hepatitis C virus 4a on patients’ response to therapy. World J Gastroenterology 15: 1480-1486
20. Kumar, M;.. and Goueli, SA (2007) A bioluminescent-based HTS-compatible assay to monitor G-protein coupled receptor modulation of cellular cyclic AMP. Assay Drug Dev. Technol. 5: 237-245
21. El-Awady,..and Goueli, SA (2006) HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro. World J Gastroenterol 12: 4836-4842
22. Kupcho,.. and Goueli, S. A.(2004) A Homogenous, Non-radioactive High-Throughput Fluorogenic Protein Phosphatase Assay. Journal of Biomol. Screening, 9(3)223-231.
23. Kelemen, B.R., Hsiao, K, and Goueli, S.A. (2002) Selective in vivo inhibition of mitogen-activated protein kinase activation using cell-permeable peptides. J. Biol. Chem., 277, 8741-8748.