307.5 - Novel insights into the fundamentals of palatal shelf elevation dynamics in normal mouse embryos

Irfan Saadi (University of Kansas Medical Center), Jeremy Goering (University of Kansas Medical Center), Michael Moedritzer (University of Kansas Medical Center), Marta Stetsiv (University of Kansas Medical Center), Dona Greta Isai (University of Kansas Medical Center), Brittany Hufft-Martinez (University of Kansas Medical Center), Zaid Umar (University of Kansas Medical Center), Madison Rickabaugh (University of Kansas Medical Center), Paul Keselman (University of Kansas Medical Center), Munish Chauhan (University of Kansas Medical Center), William Brooks (University of Kansas Medical Center, University of Kansas Medical Center), Kenneth Fischer (University of Kansas), Andras Czirok (University of Kansas Medical Center, Eotvos University)

Embryonic palate development involves bilateral vertical growth of palatal shelves – extensions from the maxillary processes – next to the tongue at embryonic day (E) 13.5. This vertical growth is followed by elevation and fusion above the tongue by E14.5. Recent models indicate that this process of elevation involves a complex vertical to horizontal remodeling of the palatal shelves. While earlier studies have implied that this is a rapid process, the precise timing has not been resolved. To further understand the dynamics of palatal shelf elevation, we employed time-restricted pregnancies with a one-hour resolution and magnetic resonance (MR) imaging of intermediate stages. Our data showed that in C57BL/6J mice, palatal shelves are unelevated in 100% of embryos at E14.0 and completely elevated in 80% of embryos at E14.25, which is only a 6-hour timeframe. Interestingly, all E14.25 embryos with unelevated palatal shelves (20%) were female, suggesting a shift towards delayed elevation in female embryos. Even at E14.125, 25% of embryos had completely elevated palatal shelves suggesting that once initiated, the palatal shelves elevated rapidly with elevation completing in less than 3 hours in individual embryos. In contrast to C57BL/6J, in FVB/NJ mice, the elevation window was shifted earlier and was broader to E13.875-E14.25 without any noticeable sex differences. In a proportion of embryos in both strains, we also captured an intermediate stage with unilateral palatal shelf elevation. Both right and left shelves were identified to be unilaterally elevated indicating that it was a random process. It remains to be determined whether palatal shelves elevate in a sequential manner, i.e., one-at-a-time, with an obligate unilateral elevation stage. MR imaging of various stages showed a highly dynamic antero-posterior remodeling of the shelves during elevation, beginning with the formation of bilateral bulges in the posterior palate. These bulges tapered anteriorly, however, palatal shelf fusion still began in the middle part of the palate. The bulge regions did not show changes in cell proliferation, consistent with the notion that rapid elevation was primarily due to cytoskeletal and extracellular matrix changes. We are using finite element analysis to identify the mechanical forces underlying these morphological changes. Thus, our data reveal critical novel insights into the rapid dynamic changes as well as strain and sex differences in palatal shelf elevation that lay the foundation for future studies of normal and abnormal palatogenesis.

This project was supported in part by the National Institutes of Health grants DE026172 (I.S.). Core support and pilot funding was provided by National Institutes of Health grants for Center of Biomedical Research Excellence (P20GM104936), Kansas IDeA Network for Biomedical Research Excellence (P20GM103418), Kansas Intellectual and Developmental Disabilities Research Center (P30HD002528), and shared instrumentation (S10OD026793).