(504.3) Lipidomics identifies novel circulating markers of CVD risk in African American and Caucasian women
Sunday, April 3, 2022
12:45 PM – 2:00 PM
Location: Exhibit/Poster Hall A-B - Pennsylvania Convention Center
Poster Board Number: A259
Paula Gonzalez (University of Wisconsin-Madison), Raghav Jain (University of Wisconsin-Madison), Chris Coe (University of Wisconsin-Madison), Carol Ryff (University of Wisconsin-Madison), Camille King (University of Wisconsin-Madison), Andy Bersh (University of Wisconsin-Madison), Kristen Malecki (University of Wisconsin-Madison), Judith Simcox (University of Wisconsin-Madison)
Cardiovascular disease (CVD) is the leading cause of mortality for women in the USA. Women with dyslipidemia have at increased risk of CVD. The parameters of dyslipidemia have primarily been defined using Caucasian male populations. Current lipid markers of dyslipidemia such as high density lipoprotein (HDL), low density lipoprotein (LDL), and triglycerides have been shown to be poor predictors of CVD risk in African American populations. We aim to identify marks of dyslipidemia in African American populations that better predict risk and development of CVD.
Untargeted lipidomics (total lipid #) was performed on sera from middle-age African American and Caucasian women (n=872). Machine learning was used to identify predictive lipid markers of CVD risk. Findings were validated using an independent cohort of women (n=200).
We identified unique and shared signatures of CVD in women based on race. Arachidonic acid containing lipids are positively correlated with elevated blood pressure and cardiomyopathy. Because these lipids are important in inflammatory signaling, we also assessed interleukin-6 and c-reactive protein. Through machine learning we were able to determine that markers of inflammation and inflammatory lipids were predictive of CVD in both Caucasian and African American women. Next, we will perform targeted lipidomics on these lipids of interest.
Regardless of class, lipid species containing arachidonic acid were increased in circulation of women with hypertension. We propose the consideration of an inflammatory marker panel that includes arachidonic acid to assess CVD risk in women.
Research reported in this publication was supported by the Eunice Kennedy Shriver National Institute of Child Health amp;amp; Human Development of the National Institutes of Health, the Office of The Director, National Institutes of Health (OD) and the National Cancer Institute (NCI) under Award Number K12HD101368. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The work was also supported in part by startup funds from the University of Wisconsin-Madison School Department of Biochemistry to J.A.S. and a Department of Biochemistry UW2020 Fellowship for P.A.G.