(504.6) Neucode Tags for Highly Multiplexed Metabolomics

Poster Board Number: A262

Scott Grady (Saint Louis University), Michael Armbruster (Saint Louis University), James Edwards (Saint Louis University), Christopher Arnatt (Saint Louis University)

We describe the design and synthesis for neutron encoded (NeuCode) tags for highly multiplexed targeted sample analysis for use in high resolution mass spectrometry (HRMS) based metabolomics. A set of aldehyde and carboxylic acid reactive tags were synthesized with a quaternary ammonium polar head group which limits off-target ionization and promotes coelution of differentially tagged metabolites. A four carbon linker allowed us to synthesize highly multiplexed NeuCode tags by providing many areas for isotope substitution and the obtained amine tags can be easily converted to other functional groups to target a wide array of metabolites. Tags were used to analyze levels of targeted metabolites in differentially treated cell lysates producing tagged samples which were mixed 1:1 and run in a single LC-MS injection.

This work was financially supported by the National Institutes of Health (lt;a href="https://www.sciencedirect.com/science/article/pii/S0003267021010862#gs1" target="_blank" rel="noopener noreferrer" data-auth="NotApplicable" data-linkindex="0"gt;5R01GM134081lt;/agt;).