(625.7) Subcellular Protein Expression of Annexin A1 (ANXA1) in Different Types of Cancer

Poster Board Number: E671

Karyn Olascuaga-Castillo (Universidad Nacional de Trujillo), Susana Rubio-Guevara (Universidad Nacional de Trujillo), Elena Cáceres-Andonaire (Universidad Privada Antenor Orrego), Olga Caballero-Aquiño (Universidad Nacional de Trujillo), Elena Mantilla-Rodríguez (Universidad Nacional de Trujillo), Julio Hilario-Vargas (Universidad Nacional de Trujillo), José Morgado-Díaz (Instituto Nacional de Cancer), Maxim Berezovski (University of Ottawa)

Annexin A1 (ANXA1) is a Ca2+-regulated phospholipid-binding protein involved in several physiological functions. Further research has shown that this protein might have various roles in cancer development and operate at different levels. This is partially related to the placement of ANXA1 in distinct cellular compartments. ANXA1 is maybe nuclear, cytoplasmic, and membrane-associated. The goal of this study has been to analyze ANXA1 subcellular localization and expression in different types of cancer.

Methods: We examined 1295 data sets available in The Human Protein Atlas (HPA) (HPA). The research examined the ANXA1 expression in tissue from 1130 cancer and 165 normal samples. A visual categorization method based on staining intensity (I), the proportion of stained cells (F) (F). ANXA1 expression levels as a function of subcellular localization.

Q Score (product of E*I) was calculated by the fraction of stained cells (F) and the intensity (I) (I)

Results: In cancer patients, the predominant subcellular localization was cytoplasmic membranous (53.3 %), whereas, in healthy individuals, the principal was cytoplasmic membranous and nuclear (57.0 %). In terms of staining intensity (Q Score), the highest values were found at the cytoplasmic membranous and nuclear levels (Q Score=5.69). In contrast, the highest Q Score values were found in standard samples at the nuclear level (Q Score=6.0).

Conclusion: The subcellular localization of ANXA1 expression may be a helpful diagnostic marker in various cancer types.

This research was funded by the Doctoral Program in Pharmacy and Biochemistry of the National University of Trujillo. Peru and the National Council of Science and Technology - Peru (CONCYTEC) in cooperation with the World Bank (Contract N 07-2018-FONDECYT-BM-IADT-MU).