(598.3) Study on exosome proteomics related to Parkinsons disease

Poster Board Number: E412

Hubin Duan (The First Hospital of Shanxi Medical University, The First Hospital of Shanxi Medical University, The First Hospital of Shanxi Medical University), Chunyan Hao (The First Hospital of Shanxi Medical University), Mingli Li (The eleventh clinical college of Shanxi Medical University), wen Xue (The eleventh clinical college of Shanxi Medical University), Xiaolin Zhu (The First Hospital of Shanxi Medical University), Binjun Qiao (The First Hospital of Shanxi Medical University), Jinbao Zhang (The First Hospital of Shanxi Medical University), Xiaohong Qiao (The eleventh clinical college of Shanxi Medical University)

Background Parkinson disease (PD) is a serious neurodegenerative disease with high increasing rate. Exosomes, carrying numerous biological materials including proteins and lipids, function as crucial players in multiple diseases including PD.

 Methods  Exosomes were isolated from serum samples of PD patients and healthy people, which was validated through transmission electron microscope (TEM) image and protein expression detection of cluster of differentiation protein 9 (CD9) and tumor susceptibility gene 101 protein (TSG101) (exosome markers) via western blot assay. Differentially expressed proteins (DEPs) in serum exosomes were identified through iTRAQ-LC-MS/MS. DEPs were annotated and analyzed by Gene Ontology (GO) and KEGG Pathway databases. Protein-protein interaction networks of these DEPs were established through STRING database.

Results A total of 42 DEPs (24 up-regulated, 18 down-regulated) were identified in serum exosomes of PD patients versus healthy control group. GO-biological_process analysis showed that most of DEPs were involved in the regulation of immune system process, vesicle-mediated transport, response to stress and signal transduction. Moreover, some crucial proteins (e.g. antichymotrypsin (SERPINA3) and hemopexin (HPX)) associated with the pathology of PD were identified through interaction analysis. Western blot and ELISA assays further validated that SERPINA3 and HPX were highly expressed and phospholipid transfer protein (PLTP) was low expressed in serum exosomes of PD patients versus control group.

Conclusion Proteomics analysis for serum exosomes of PD patients and healthy people could provide some potential biomarkers and vital information of intercellular communication and pathogenic mechanisms in PD.

Key words  Parkinson disease, proteomics, iTRAQ, mass spectrometry.

Support or Funding Information

This study was supported by the Shanxi Scholarship Council of China, Department of Human Resources and Social Security of Shanxi province, Scientific research Project of Shanxi Provincial Health Commission，The Shanxi Province Basic Research Program, The Education Reform Project of Shanxi Medical University, Lvliang Science and Technology Bureau scientific research program，Foundation of the eleventh Clinical Medical College of Shanxi Medical University.