(767.1) HH pathway orchestrates the epithelial-mesenchymal crosstalk during human lung branching morphogenesis

Poster Board Number: E579

Randa Belgacemi (Lundquist Institute for Biomedical Innovatio), Soula Danopoulos (Lundquist Institute for Biomedical Innovatio), Gail Deutsch (Pediatric Molecular and Personalized Medicine Program and Division of Neonatology), Valerian Dormoy (Inserm UMR-S1250, P3Cell), Denise Al Alam (Lundquist Institute for Biomedical Innovatio)

Rationale:

Hedgehog (HH) pathway plays an essential role in lung branching morphogenesis in rodents. However, little is known about the role of this pathway in human lung development. We hypothesize that HH pathway is necessary for branching during human lung development and hence in pulmonary hypoplasia.

Methods:

Expression of HH pathway components was analyzed in single cell and bulk RNA-sequencing data from publicly available lung datasets. Fresh human fetal lung tissues between gestational ages 10-18 weeks were collected. RT-qPCR, fluorescent in situ hybridization (FISH) and immunofluorescence staining (IF) were performed to determine spatiotemporal localization of HH pathway actors. In addition, distal lung segments were cultured in air-liquid interface for up to 48 hours in the presence or absence of HH inhibitor (5E1). RT-qPCR, western blot and IF were performed to investigate the effect of HH pathway inhibition on epithelial-mesenchymal markers and associated signaling pathways.

Results:

Bulk and scRNA-seq analysis showed an early expression of HH pathway components during human lung development. We noted an inverse expression between activators and inhibitors receptors (PTCH1/HHIP) over time. PTCH1 had a higher expression at 20 weeks compared to 14 weeks while the opposite was observed for HHIP. HHIP FISH staining was restricted to ACTA2+ airway smooth muscle cells while PTCH1 was primarily localized in mesenchymal populations adjacent to the tip of airway epithelial buds where SHH ligand is produced. Inhibiting HH signaling ex vivo reduced branching during development (2.5-fold vs control, plt;0.01, n=8). Consequently, SOX9 expression, an epithelial progenitor marker, was increased whereas ACTA2 was decreased (respectively 1.5- and 2-fold vs control, plt;0.05, n=8). These results were confirmed by western blot and IF staining. No change was observed for SOX2 gene expression while a significant increase in protein expression was observed. FGF10 increased following HH inhibition (1.5-fold vs control, plt;0.01, n=8), but the expression of ETV4 and 5 decreased (1.5-fold vs control, plt;0.05, n=8). Finally, we demonstrated that HH signaling elements (including SHH, PTCH1, GLI2) were downregulated in lung tissues from congenital diaphragmatic hernia patients.

Conclusion:  

We have determined for the first-time gene expression and localization of HH pathway during human lung morphogenesis. Moreover, we showed that HH signaling inhibition alters important genes and proteins expression, such as epithelial-mesenchymal progenitors and associated pathways, required for proper branching processes. Understanding the role of HH pathway on human lung development could lead to the identification of novel therapeutic targets for childhood pulmonary diseases.