(771.5) Mitochondrial calcium retention capacity correlates with markers of muscle mass, strength, power and functional capacities in young and old adults

Poster Board Number: E611

Marina Cefis (Université du Québec à Montréal), Vincent Marcangeli (Université du Québec à Montréal), Jordan Granet (Université du Québec à Montréal, Centre de Recherche de l’Institut Universitaire de Gériatrie de Montréal), Rami Hammad (Université du Québec à Montréal, Centre de Recherche de l’Institut Universitaire de Gériatrie de Montréal), Jean-Philippe Leduc-Gaudet (Venetian Institute of Molecular Medicine), Pierrette Gaudreau (Centre de Recherche du Centre Hospitalier Universitaire de Montréal), Richard Robitaille (Université de Montréal), Marc Belanger (Université du Québec à Montréal), Mylène Aubertin-Leheudre (Université du Québec à Montréal, Centre de Recherche de l’Institut Universitaire de Gériatrie de Montréal), José Morais (McGill University, Research Institute of the McGill University Health Center), Gilles Gouspillou (Université du Québec à Montréal, Centre de Recherche de l’Institut Universitaire de Gériatrie de Montréal, McGill University)

Rationale: The aging-related loss of muscle mass and function is a major risk factor for falls, fractures, frailty, loss of independence, institutionalization and even death. Although the underlying cellular and molecular mechanisms remain partly understood, impaired mitochondrial permeability transition pore (mPTP) function has been proposed as a potential mechanism. Indeed, altered mitochondrial calcium retention capacity (mCRC), a marker of mPTP function, has been documented with aging in both rodent and human skeletal muscles and mPTP opening was shown to cause muscle atrophy. However, studies on the impact of aging on mPTP function in humans remain scarce, and whether impaired mPTP function correlates with markers of muscle mass, performance and functional capacities remain unknown.

Objective:  The present study aimed at investigating whether mCRC is associated with markers of muscle mass, muscle function and functional capacities in humans.

Methods:  Twelve young (29.4 ± 4.4y) and fourteen old (80 ± 7.4y) healthy men were enrolled in the present study. Leg lean mass (leg LM) was assessed using DEXA. Maximal isometric knee extension strength (iKES) and power were measured using a strain gauge system attached to a chair and the Nottingham Leg Extensor Power rig, respectively. To assess functional capacities, the performance at the 6-min walk test and the step test (maximum number of steps in 20s) were evaluated. To assess mPTP function, mCRC was measured in permeabilized myofibers prepared from muscle biopsies obtained in the vastus lateralis.

Results:  As compared to young adults, old individuals displayed significantly lower leg LM (plt;0.01), iKES (plt;0.01), and power (plt;0.01). The performance at the 6-min walk test (plt;0.01) and the step test (plt;0.01) were also lower in old individuals. Old individuals also displayed a significantly lower mCRC (plt;0.01). mCRC was significantly correlated with leg LM (r=0.34; p=0.045), iKES (r=0.35; p=0.04) and muscle power (r=0.41; p=0.02). In addition, mCRC was significantly correlated with the performance at the 6-min walking test (r=0.42;p=0.01) and step test (r=0.46; plt;0.01).

Conclusions:  These results indicate that mPTP function is altered with aging in humans and highlight positive associations between mPTP, muscle mass, strength, power, and functional capacities. These findings therefore strengthen the emerging view that altered mPTP function contributes to the aging-related loss of muscle mass and function in humans.

This study was funded by a CIHR grant (GER417022). GG is supported by a Chercheur Boursier Junior 2 salary award from the FRQS. JPLG is supported by a FRQS postdoctoral fellowship.