(817.10) Evaluation of the antioxidant effects of green synthesis silver nanoparticles on brain from rats with experimental diabetes

Poster Board Number: A410

Jenaro Lemus-de la Cruz (Universidad Michoacana de San Nicolás de Hidalgo), Cinthia Landa-Moreno (Universidad Michoacana de San Nicolás de Hidalgo), Mitchell Trejo-Hurtado (Universidad Michoacana de San Nicolás de Hidalgo), Donovan Peña-Montes (Universidad Michoacana de San Nicolás de Hidalgo), Rafael Salgado-Garciglia (Universidad Michoacana de San Nicolás de Hidalgo), Salvador Manzo-Avalos (Universidad Michoacana de San Nicolás de Hidalgo), Christian Cortés-Rojo (Universidad Michoacana de San Nicolás de Hidalgo), Rocío Montoya-Pérez (Universidad Michoacana de San Nicolás de Hidalgo), Alfredo Saavedra-Molina (Universidad Michoacana de San Nicolás de Hidalgo)

Diabetes mellitus (DM) is a metabolic disorder of multiple etiology, the main characteristic is that occurs with chronic hyperglycemia, due to insulin deficiency or resistance, or a combination of both. One of the organs most affected by the complications of DM is the brain because DM is closely related to an increase in the production of reactive oxygen species (ROS), an imbalance of the cellular antioxidant defense system and mitochondrial dysfunction. It has been reported that the brain is highly susceptible to oxidative stress. With the course of the disease, there are various complications at the brain level, such as the development of Alzheimers, cognitive decline, alterations in neurotransmission, neuroinflammation, apoptosis, and necrosis, among others. On the other hand, traditional medicine has emerged as an alternative in the treatment of various diseases, including DM; one of the plants used in traditional medicine to treat diabetes is Eryngium carlinae, which has already been experimentally shown to have hypoglycemic, antioxidant and hypolipidemic properties, and can be used as an antidiabetic treatment. Moreover, new strategies have been sought to ensure a rapid and effective administration of drugs in recent years. One of these strategies is using green synthetic nanoparticles, which combine the use of plant extracts with metallic nanoparticles that ensure a more significant biological effect. The objective of this work was to compare the effect of the extract and its combination with silver nanoparticles (AgNP) on glucose levels and mitochondrial function. AgNP was obtained from the aqueous extract of E. carlinae characterized by UV-Vis and SEM. 36 male Wistar rats without or with experimental DM (STZ 45 mg/Kg of weight) were divided into 6 groups with n = 6 and were treated or not with a dose of 30 mg/Kg of weight of the aqueous extract of E. carlinae or with a dose of 30 mg/Kg of weight of the extract-AgNP combination for 45 days. The diabetic group showed a significant increase in glucose and triglycerides in the blood, as well as the production of ROS and lipoperoxidation in mitochondria. Also, a decrease in SODmt, GPx and complexes I, II and III activities compared to the control group. Administration with the treatments in diabetic rats showed decreased ROS production and lipoperoxidation, they also restored the activities of SODmt, GPx and CTE complexes compared to the diabetic group, and only the group treated with the extract-AgNP combination decreased significantly the blood glucose and triglyceride levels, compared to the diabetic group. Our results suggest that the treatment with both E. carlinae aqueous extract and the extract-AgNP combination can reverse the imbalance of the antioxidant defense system and ROS production, as well as mitochondrial dysfunction in the brain, and only with the combination (extract-AgNP) lowers glucose and triglyceride levels in rats with DM.

Support or Funding Information

The authors appreciate the partial economic support from Coordinación de Investigación Científica. UMSNH (2.16 to AS-M). JL-C, CL-M, MT-H, DP-M are CONACYT Fellows.

The authors appreciate the partial economic support from Coordinaciamp;oacute;n de Investigaciamp;oacute;n Cientamp;iacute;fica. UMSNH (2.16 to AS-M). JL-C, CL-M, MT-H, DP-M are CONACYT Fellows.