BAR (Bin, Amphiphysin and Rvs) protein domains are responsible for the generation of membrane curvature and represent a critical mechanical component of cellular function. Thus, BAR domains have great potential as components of membrane-remodeling tools for cell biologists. In this work, we describe the design and implementation of a versatile light-gated I-BAR domain containing tool (‘CRY-BAR’) with applications in the remodeling of membrane architectures and the control of cellular dynamics. By taking advantage of the intrinsic membrane association of the I-BAR domain, CRY-BAR can be used for spatial and temporal control of cellular dynamics and the initiation of cellular protrusions. CRY-BAR function is demonstrated in immortalized and primary cell lines; Ezrin, which acts as a relay between the plasma membrane and the actin cytoskeleton, is implicated as an important mediator of switch function. Overall, CRY-BAR holds promise as a widely applicable addition to the optogenetic toolkit.
ECU BMRC (Biomaterials Research Cluster) Seed Grant
