(526.8) The new in the enteral dysbiosis correction intensified by heavy metals and mycotoxins

Poster Board Number: D25
Introduction:

Vladimir Ponomarev (St. Petersburg State University of Veterinary Medicine), Oksana Popova (St. Petersburg State University of Veterinary Medicine), Viktor Baryshev (St. Petersburg State University of Veterinary Medicine)

The study aimed to research the new treatment and pharmacorrection protocol of toxical body state intensified by heavy metals and mycotoxins in the cattle using native drugs. The studies were carried out on cows aged 3 to 4 years. 2 groups were formed  (n=10 by each). The protocol of 1st group cows (n=10) was included the sorbents complex as a supplementation to the ration (4 % per the daily rate during 14th days) and the hepatoprotective agent "Hepaton-vet" (the compound: lac Carduus, ursodeoxycholic acid) at a dose of 100 ml per cow during 21 days. The protocol of 2 d group cows (n=10)  was also included the sorbents complex as a supplementation to the ration (4 % per the daily rate during 14th days) and the conventional fluid therapy. We were determined the sorbent net efficiency as a quantity characteristic of loading capacity. The sorption of mycotoxins was indicated by the quantity-related method under different pH, which was affected the pH replacement of the gastrointestinal tract in animals. The heavy metals in milk and feed samples were analyzed by the periodate method and atomic adsorption spectrometry. The level of mycotoxins was tested by the Agra quant ELISA method. We were used the software program  "Statistica 6" with Students t-test for dependent adjectives as a statistical analysis of results. The highest level of cadmium was contained in the dry forage samples (0.14±0.05 mg/kg). The difference of their between the dry forage and mixed feed with silage was 27 %.  The results of the biochemical analysis were showed hypoproteinemia and hypoalbuminemia (21.23±1.12 g/l). The reason could be the dystrophic change of hepatocytes and the deranged biosynthesis of this fraction with the shift to γ-globulins increasing. The increase of albumin level was on the 12th day of treatment (29.19±1.32 g/l). The albumin level of cows 1st group was increased by 21.2 % and 17.3 % consequently by the end of the experiment compared with the value of cows control group. Y-globulins were decreasing by 37.3 % and 40.5 %. The atmospheric methane emissions in the 2d group were decreased by 8 % (84±3.5 mg/m3). So, we can assume that using the complex of sorbents with hepatoprotective agent "Hepaton-vet" has a positive influence on the livers metabolic process that has evidenced results of morpho-biochemical analysis and clinical diagnostic of animals.