(760.3) Timeline of Multi-organ Plasma Extravasation After Bleomycin-Induced Acute Lung Injury

Poster Board Number: E498

Oliver Kitzerow (University of Nebraska Medical Center), Irving Zucker (University of Nebraska Medical Center), Steven Lisco (University of Nebraska Medical Center), Hanjun Wang (University of Nebraska Medical Center)

Acute lung injury (ALI) is characterized by the abrupt onset of clinically significant hypoxemia with the presence of pulmonary edema. ALI is associated with cytokine release and plasma extravasation (PE) that can cause pulmonary edema and subsequently acute respiratory distress syndrome (ARDS). Therefore, it is critical we understand the relationship between ALI and lung PE. In addition, it is also important to assess PE in the lungs and other organs post-ALI since ALI/ARDS often causes multi-organ failure. We hypothesized that ALI induces time-dependent lung PE, which promotes extravasation in the heart, liver, kidney, spleen, pancreas, and gastrointestinal (GI) tract, in a time-dependent manner. To test our hypothesis, we administered bleomycin or saline via tracheal intubation in 8-week-old Sprague Dawley rats to create ALI. At the terminal experiments, Evans Blue was injected (IV) through the femoral vein to allow for the visualization of PE. Plasma extravasation of desired organs was evaluated at 3-, 7-, 14-, 21-, and 28-days after bleomycin or saline treatment by evaluating Evans Blue concentrations calorimetrically at the absorption maximum for Evans blue (620 nm). Data show that ALI induces lung PE beginning at day 3 and peaking between 7 and 21 days. Extravasation can also be seen in all organs at varying degrees beginning at day 3 and peaking between day 7 and 14. Resolution appears to start after day 21 and continues past day 28. We conclude that ALI caused by bleomycin incites a time-dependent PE of the lungs and multiple other organs.

This study was supported by NIH grant lt;a href="https://public.era.nih.gov/grantfolder/viewCommonsStatus.era?encryptedParam=(v2)ETMsDgAAAWy1o8xtABRBRVMvQ0JDL1BLQ1M1UGFkZGluZwCAABAAEIyB9u4jyNg8UCkPYqhOgTUAAAAQ9lAL84yF7gnnjMzsv2KoKwAUch1O5EEf-qQlul-evudmrCQP0gk." target="_blank"gt;R01 HL-152160lt;/agt; and in part, by NIH grants R01 HL-121012 and R01 HL126796. Dr. Hanjun Wang is also supported by Margaret R. Larson Professorship in Anesthesiology.