(713.7) Comparison of del Nido and Histidine-tryptophan-ketoglutarate cardioplegic solutions on cardiac and endothelial function

Poster Board Number: E51

Hong-Mei Xue (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences, Department of Physiology, Hebei Medical University), Hai-Tao Hou (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences), Wen-Tao Sun (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences), Shi-Fu Wang (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences), Shan Guo (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences), Qin Yang (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences), Guo-Wei He* (The Institute of Cardiovascular Diseases amp; Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tianjin University amp; Chinese Academy of Medical Sciences , Drug Research and Development Center, Wannan Medical College, Department of Surgery, Oregon Health and Science University)

Objectives: This study was designed to compare cardiac and endothelial protection of three clinically used cardioplegias del Nido (DNC), Histidine-tryptophan-ketoglutarate (HTK), and cold blood (BC) cardioplegia followed by HTK (BC+HTK) in ischemia/reperfusion（I/R）rat model.

Methods: Sixty male Wistar rats were subjected to either 120-min global ischemia at 4°C followed by 90-min reperfusion at 37°C or no I/R (Control) in Langendorff apparatus and were randomly allocated into five groups: Control, I/R, DNC, HTK, and BC+HTK. Hemodynamic parameters were continuously recorded. The endothelium-dependent relaxation to acetylcholine (ACh) was measured in the left anterior descending artery thereafter in myograph. The protein expression of cardiac Troponin T (cTnT) and creatine Kinase MB (CKMB) were measured by western blotting.

Results: During reperfusion, HTK had higher LVSP whereas DNC had lower LVEDP, better LVDP, and best +dp/dtmax and -dp/dtmax than other two groups but the differences disappeared at the end of the reperfusion. HTK or BC+HTK preserves the ACh-induced endothelium-dependent relaxation better than DNC (Emax=48.2±8.0% in DNC vs. 75.0±8.0% in HTK, plt;0.05; vs. 96.9±3.5% in BC+HTK, plt;0.001). The protein level of cTnT and CKMB was downregulated in the three groups.

Conclusions: DNC shows a better recovery in diastolic function, whereas HTK or BC+HTK exerts better protection in coronary endothelial function. All these three cardioplegias could ameliorate the myocardium injury at the protein level. These findings may suggest that searching for “perfect” cardioplegia is warranted.

lt;igt;Supported by the National Natural Science Foundation of China [82170353 amp;amp; 81870288 amp;amp; 81870227]; the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2020-PT310-007 amp;amp; 2019XK310001]; Tianjin Binhai New Area Health Commission [2019BWKY011]; Tianjin Science and Technology Project [18PTZWHZ00060]; TEDA International Cardiovascular Hospital[2019-TD-003].lt;/igt;