Session: 673 Oxidative stress and reactive oxygen I
(673.9) The Effect of Mitochondrially Targeted Antioxidants on Wound Healing of Human Dermal Fibroblasts
Monday, April 4, 2022
12:30 PM – 1:45 PM
Location: Exhibit/Poster Hall A-B - Pennsylvania Convention Center
Poster Board Number: A403
Jessica Carney (Bemidji State University), Megan Flateland (Bemidji State University), Brynn Hetland (Bemidji State University), Kevin Renquist (Bemidji State University), Kjerstin Owens (Bemidji State University)
Oxidative stress is known to be a destructive result of wounded tissues and is thought to be a source of delayed wound healing; however, the relationship between reactive oxygen species (ROS) and wound repair is not completely known. We hypothesized that the use of mitochondrially targeted antioxidants will increase the rate of wound healing of normal skin fibroblasts by decreasing steady-state levels of reactive oxygen species at the wound site. Scratch assays showed that ROS accumulation was prominent at the wound site and increased overtime several hours. Cells were treated with mitochondrially targeted antioxidants and their nontargeted counterparts. Treatment with antioxidants decreased ROS levels in wounded and unwounded cells and increased the rate of wound healing. Finding a correlation between ROS and wound healing and how they are affected by antioxidants will offer an increased understanding of the biological process of wound healing and may propose therapeutic options to promote healing efficacy.
Support or Funding Information
Portions of this project were supported by the Bemidji State University New Faculty Grant and the Beitzel Biochemistry Student Research Fund.