(826.6) Altered gut microbiome structure and its association with inflammation markers in patients with Crohn’s disease in comparison to healthy siblings

Poster Board Number: D24

Jun Hu (The Sixth Affiliated Hospital, Sun Yat-sen University), Sijing Cheng (The Sixth Affiliated Hospital, Sun Yat-sen University, The Sixth Affiliated Hospital, Sun Yat-sen University), Wanning Chen (School of Life Sciences and Technology, Tongji University), Jiayin Yao (The Sixth Affiliated Hospital, Sun Yat-sen University), Yichen Li (The Sixth Affiliated Hospital, Sun Yat-sen University), Wenxia Wang (The Sixth Affiliated Hospital, Sun Yat-sen University), Yibo Huang (The Sixth Affiliated Hospital, Sun Yat-sen University), Lixin Zhu (The Sixth Affiliated Hospital, Sun Yat-sen University), Ruixin Zhu (School of Life Sciences and Technology, Tongji University), Min Zhi (The Sixth Affiliated Hospital, Sun Yat-sen University)

Background:

Previous studies reported inconsistent results regarding altered gut microbiome in patients with Crohns disease (CD), likely due to the large impact of genetic polymorphisms and environmental factors on the structure of the gut microbiota. We aim to investigate the altered microbiome in CD with a cohort that minimizes the impact of irrelevant genetic and environmental factors on the gut microbiota.

Method:

Stool samples and clinical records were collected from 91 patients withCD and their healthy siblings in the Sixth Affiliated Hospital of Sun Yat-sen University in Guangzhou, China, between March 2014 and December 2018. Fecal DNA was isolated and the V5-V6 region of the 16S rRNA gene was sequenced on a MiSeq platform. Data was processed with QIIME2. Spearman analysis was performed to identify the microbial features correlated with inflammatory markers.

Result:

Compared to healthy siblings, CD patients exhibited reduced alpha diversities based on the metrics of Chao1, Shannon, and observed speciesat the ASV level (Plt;0.05). Beta diversity was significantly different between study groups according to UniFrac based principle coordinate analysis (Plt;0.001). Altered abundances were observed at various taxonomic levels comparing between CD patients and their healthy siblings, including increased abundance in Escherichia-Shigella and Veillonella, and decreased abundance in Lachnospiraceae, Prevotella 9 and Faecalibacterium in CD patients. Spearman analysis identified significant correlations between Escherichia- Shigella and Lachnospiraceae with inflammatory markers erythrocyte sedimentation rate, C-reactive protein, and white blood cell count （Fig.1）.

Discussion: By comparing between CD patients with their siblings, the impact of the compounding genetic and environmental factors on the gut microbita was minimized. This approach allowed the identification of CD specific microbial fearures that are correlated with inflammatory markers. Further study along this line may establish CD specific microbial targets for diagnostic and therapeutic purposes.

lt;olgt;lt;ligt;the Sun Yat-Sen University Clinical Research 5010 Program（2014008）lt;/ligt;lt;ligt;National Natural Science Foundation of China 81770571, 81774152, 82170542.lt;/ligt;lt;ligt;Guangdong Province amp;ldquo;Pearl River Talent Planamp;rdquo; Innovation and Entrepreneurship Team Project 2019ZT08Y464.lt;/ligt;lt;/olgt;



Figure1. Correlation of differential taxa with inflammatory markers in Crohn's disease