(675.3) The Effects of Prune Extract on Cellular Models of Bone Cancer

Poster Board Number: A415

Chelsie Miller (San Diego State University), Mowaffaq Adam (San Diego State University), Grace Chao (San Diego State University), Shirin Hooshmand (San Diego State University), Christal Sohl (San Diego State University)

There is strong evidence for prunes as a health food for improvement in digestion due to their high fiber content. More recent work has shown that prunes also have a positive impact on bone health, attributed in part to the antioxidant compounds and polyphenols they possess. Despite their important antioxidant role, the anticancer properties of prunes has yet to be determined. We hypothesize that treatment of osteosarcoma cell lines with prune extracts will lead to a decrease in cell proliferation and migration, driven at least in part due to the antioxidant compounds. We will treat C28, Saos-2, and hFOB 1.19 bone cell lines with varying concentrations of prune extract, and use the CellTiterGlo assay and scratch assays to measure the effects on cell proliferation and migration, respectively. A key component of our work is establishing the optimum cell culturing conditions and developing robust protocols for important cancer phenotype assays. Upon completion of these experiments, we hope to better understand the effects of prunes on bone health and disease.

Support or Funding Information

This work was funded by a Research Scholar Grant, RSG-19-075-01-TBE, from the American Cancer Society (C.D.S.), National Institutes of Health R35 GM137773 (C.D.S.), and IMSD 5R25GM058906 (SDSU), as well as the California Metabolic Research Foundation (SDSU).