(815.2) Increased Fatty Acid Synthesis and Catabolism Supports Metastatic Breast Cancer Cell Migration

Poster Board Number: A381

Chaylen Andolino (Purdue University ), Dorothy Teegarden (Purdue University)

Lipid accumulation is positively associated with breast cancer aggressiveness and poorer clinical outcomes. Despite this observation, mechanisms underlying the increase and role of stored lipid in breast cancer progression remain incompletely understood. The accumulation of triacylglycerol (TAG) is dependent on the balance of uptake, synthesis, and utilization of fatty acids (FAs). In the current study, we utilized non-metastatic MCF10A-ras and metastatic MCF10CA1a human breast cancer cells to determine differences in TAG storage and catabolism for sustaining migration—a critical step in the metastatic cascade. Our results demonstrate that MCF10CA1a cells have 90% more TAG than MCF10A-ras (TAG Assay and Transmission Electron Microscopy), although no significant difference in 14C-palmitate uptake. MCF10CA1a cells have greater FA synthesis as shown by greater incorporation of 13C-acetate (60%), 13C-glucose (65%), and 13C-glutamine (50%) into palmitate compared to the MCF10A-ras cells (LC-MS/MS), as well as higher protein levels of FA synthase (FASN). Additionally, MCF10CA1a cells display greater flux of uniformly-labeled 13C-glucose and 13C-glutamine to the FA synthesis precursor, citrate, and lower intracellular citrate pool size compared to MCF10A-ras. In addition to increased FA anabolism, MCF10CA1a cells rely on FA oxidation (FAO) for cellular migration compared to MCF10A-ras cells, as determined by inhibiting the rate-limiting enzyme of FAO, carnitine palmitoyltransferase 1, with etomoxir (transwell and wound-healing assays). Pretreatment with the FASN inhibitor TVB-3166 (3 d) significantly reduced TAG levels and subsequent migration by 67% compared to vehicle-treated cells. Similarly, pretreatment with inhibitors of the TAG-synthesizing enzymes diacylglycerol O-acyltransferase 1 and 2 reduced TAG accumulation and subsequent migration (75%) of MCF10CA1a cells compared to vehicle. Together, the data suggest that FA synthesis may contribute to TAG storage necessary to sustain breast cancer migration. To test this hypothesis, we inhibited the initial enzyme of the TAG lipolysis pathway, adipose triacylglycerol lipase (ATGL; inhibitor ATGListatin), and measured migration. Inhibition of ATGL alone reduced MCF10CA1a cell migration (20% decrease compared to vehicle), and addition of the FAO inhibitor (etomoxir) provided no further effect. Our study indicates that metastatic MCF10CA1a cells accumulate FAs by increasing de novo lipogenesis, store them as TAG, and that catabolism of these stores drives FAO-dependent migration.

Support or Funding Information

Support from Purdue University Center for Cancer Research, Purdue University Electron Microscopy Facility, Purdue University Metabolite Profiling Facility, NIH 5R01CA232589, Indiana Clinical Translational Science Institute NIH/NCRR Grant #TR000006.



Breast Cancer Model.  Metastatic MCF10CA1a cells have a significantly higher level of triacylglycerol and cytoplasmic lipid droplets (CLD) compared to non-metastatic MCF10A-ras cells. Proposed Model.  Increased fatty acid synthesis in the metastatic breast cancer cell line, MCF10CA1a, promotes storage of triacylglycerol from increased de novo lipogenesis, functioning as a reservoir of stored lipid to be catabolized via fatty acid oxidation for energy production to support cellular migration.