OBJECTIVE：MTDH has been identified to play a critical role in many cancer types, but its potential mechanism in Non-Small Cell Lung Cancer (NSCLC) still remains unclear. We investigated the role of miRNA30a-5p in the regulation of MTDH expression, which is known as an essential biomarker for NSCLC initiation and progression. MATERIALS AND METHODS： Quantitative real-time PCR (qRT-PCR) has been performed to evaluate the expression level of MTDH and miR30a-5p. Then, we transfected miR30a-5p mimic and inhibitor into H1299 and A549 cells to investigate their impact in cell proliferation, migration, and invasion by using CCK-8 assay, wound-healing assay, and transwell assay respectively. The evidence of miR30a-5p as a specific target of the MTDH gene was explored through luciferase report assay. The expression level of MTDH was evaluated by its mRNA and protein level using a western blot assay. RESULTS：Results revealed that miR30a-5p was upregulated whereas MTDH was downregulated. Down-expression of miR30a-5p increases the expression level of MTDH and promotes NSCLC cell proliferation and invasion. Luciferase report assay confirmed the binding of miR30a-5p on MTDH mRNA 3UTR region. CONCLUSIONS：This funding highlights the silencing mechanism of MTDH in lung cancer and further revealed that MTDH can be used as a potential diagnostic and therapeutic biomarker.
This study was supported by grants of Zhongnan Hospital of Wuhan University .Research amp;amp; Development special cultivation project（Grant No: znyp2017054) and the joint Funds of Hubei Provincial Health Commission(Grant No: WJ2019H034).
