Objective: In our focus regarding microRNA, single-stranded, small non-coding RNAs, we aim to understand miRNAs roll and functional importance concerning congestive heart failure (CHF) and myxomatous mitral valve degeneration (MMVD). Characterizing expression profiles of circulating microRNAs via genome-wide sequencing for dogs with CHF secondary to MMVD.
Animals: 8 healthy client-owned dogs and 8 middle age-matched client-owned dogs with CHF secondary to MMVD.
Procedures: Blood samples were collected before administering cardiac medications for the management of CHF. Quantitative reverse transcription PCR (qRT-PCR) assays were used to validate expression profiles of differentially expressed circulating microRNAs identified from NGS analysis of dogs with CHF.
Conclusion and Clinical Relevance: Awaiting data indicating the patterns of microRNA expression are distinctive and denote molecular biomarkers with CHF in dogs with MMV.
