PD21-07: Obesity Influence on Bladder Inflammation: A Cystitis Model

Cristiano T. de Andrade, Marina Z. C. dos Santos, Guilherme Z. Rocha, Karen L. Ferrari, Campinas, Brazil, Rodolfo B. dos Reis, Ribeirão Preto, Brazil, Leonardo Reis*, Campinas, Brazil

Introduction: Recently the role of subclinical inflammation in obesity gained prominence. The association between obesity and chronic inflammation has been observed in several studies that show the relationship between increased morbidity and high Body Mass Index (BMI). This study aims to compare inflammatory pathways in obese and non-obese mice after the exposure to a pro-inflammatory carcinogen as a cystitis model.

Methods: We divided 16 females, 7 weeks old mice in two groups: 1) CONTROL: submitted to a standard diet, and 2) OBESE: submitted to a high fat diet (HFD) for 8-weeks. Both groups underwent a protocol for MNU carcinogen bladder instillation. Bladder was analyzed by histopathology, western blotting of proteins phosphorylation associated with the inflammatory pathway (JNK, JUN, NF?B, IKK) and apoptosis and proliferation co-localization by immunohistochemistry.

Results: Most OBESE mice (62.5%) presented moderate chronic active inflammation in the submucosa and mild submucosal hyalinization and 37.5% presented moderate chronic active inflammation in the submucosa and diffuse muscle layers. Most CONTROL mice (75%) had no histological alteration in bladder tissue and 25% had hyalinization of the submucosal layer. Furthermore, OBESE mice had a high expression of NF?B and higher apoptotic (0.35 vs. 0.14) and proliferative indexes (0.52 vs. 0.17).

Conclusions: Cystitis might exacerbate in obese mice which represents a chronic inflammatory state, and could be associated with an increased risk for urinary tract infection and pyelonephritis in obese patients.

Source of Funding: None