Session/Event: Energy and Macronutrient Metabolism (Oral 12)

(OR12-05-22) Postprandial and Fasting Metabolic Signatures: Insights From the ZOE PREDICT 1 Study

On Demand

Presenting Author(s)

Inbar Linenberg, MSc

Staff
King's College London
London, England, United Kingdom

Disclosure: Disclosure(s): Zoe Ltd: Employee (Ongoing)

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Co-Author(s)

KB

Kate M. Bermingham, Phd

Post Doctoral Research Assistant
Kings College London
London, England, United Kingdom
MM

Mohsen Mazidi, PhD

King's College London
London, England, United Kingdom
AV

Ana Valdes, PhD

University of Nottingham
nothingaam, England, United Kingdom
PF

Paul Franks, PhD

Lund University
Malmo, Dalarnas Lan, Sweden
AC

Andrew Chan, MD, MPH

Professor of Medicine
Massachusetts General Hospital
Boston, Massachusetts, United States
JW

Jonathan Wolf, PhD

ZOE Global
London, England, United Kingdom
Tim D. Spector, MD

Director, TwinsUK
King's College London
London, England, United Kingdom
Jose M. Ordovas, PhD

Director, Nutrition and Genomics
Tufts University
Boston, Massachusetts, United States
WH

Wendy Hall, BSc, PhD

Reader
King's College London
London, England, United Kingdom
Sarah E. Berry, RD

Senior Lecturer
King's College London
London, England, United Kingdom

Objectives: Postprandial metabolomic signatures, although not well characterized, may provide greater insight into individuals’ responses to food and subsequent cardiometabolic disease risk compared to fasting and routine clinical measures. Using the PREDICT 1 cohort, we assessed postprandial changes and inter-individual variability in metabolites sequenced by NMR.

Methods: The ZOE PREDICT 1 study (n = 1,002 healthy UK adults; NCT03479866) measured 250 metabolite parameters (Nightingale Health NMR panel, related to lipids, amino acids, glycolysis, ketones, and glycoprotein acetyls (GlycA)) by venous cannulation at fasting and postprandially after a mixed nutrient sequential test meal (4 and 6 h after meal 1, 3.7 MJ; meal 2 given at 4 h, 2.2 MJ). Postprandial changes in metabolites and their inter-individual variability (median absolute difference from the median (MADM)/median (%)) were evaluated. Associations (Spearman's correlations) and differences in variances (Fligner-Killeen test) were assessed between fasting and postprandial (6 h) measures.

Results: A significant 6 h postprandial change from fasting was seen in 85% of metabolites; of which, 47% increased, and 53% decreased (Kruskal-Wallis p< 0.05 for all). Ketone bodies and very-large lipoprotein particles showed the greatest changes. Fasting and postprandial measures had large, yet similar, inter-individual variability (MADM/median; 15% at 0, 4 and 6 h (mean for all)) and were strongly correlated (r > 0.8; 71% of measures), although ketone bodies, glucose, branched chain amino acids and LDL diameter were only weakly correlated (r < 0.5). Inter-individual patterns of response differed postprandially compared to fasting (Fligner-Killeen test of variance, p< 0.05).

Conclusions: In this large and generally healthy cohort, we demonstrate significant changes in circulating metabolites between the fasting and postprandial phase, as expected, within lipoprotein size and composition remodelling, glycolysis, essential amino acid and ketone body pathways. The large inter-individual variability in postprandial metabolite levels, suggests dietary challenges offer an opportunity for stratifying metabolic responses.

Funding Sources: ZOE Ltd