(PO25-45-22) Mixed Nuts as Healthy Snack: Effect on Gut Microbiota and Tryptophan Metabolism

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Presenting Author(s)

JY

Jieping Yang, PhD
– Associate Project Scientist, University of California, Los Angeles, Los Angeles, California, United States

Co-Author(s)

AH

Albert Hsu
– University of California, Los Angeles
JP

Jonathan Pai
– University of California, Los Angeles
SY

Scarlet Yang
– University of California, Los Angeles
SH

Susanne M. Henning
– University of California, Los Angeles
RL

Rupo Lee, MS
– Center for Human Nutrition, David Geffen School of Medicine at UCLA, Los Angeles, California, United States
JH

Jianjun Huang, BS
– Research Assistant, University of California, Los Angeles, Los Angeles, California, United States
DH

David Heber, MD, PhD
– Professor Emeritus, University of California, Los Angeles, Los Angeles, California, United States
ZL

Zhaoping Li, MD, PhD
– Professor, University of California, Los Angeles, Los Angeles, California, United States

Disclosure(s):

Jieping Yang, PhD: No relevant financial relationship(s) with ineligible companies to disclose.

Objectives: We recently demonstrated that the consumption of mixed tree nuts (MTNs) as part of a hypocaloric diet decreased heart rate and increased satiety. MTNs can be categorized as a high tryptophan (Trp) food and disturbed Trp metabolism has been associated with cardiovascular disease. The fiber and polyphenol content of MTNs have been suggested to affect human health in part through the gut microbiome. Here, we aim to investigate the effect of MTNs on Trp metabolism and the gut microbiome and the link to cardiovascular markers and satiety.

Methods: Stool and plasma samples at baseline, week 12 and 24 from our recent randomized, controlled, two-arm study were studied. In the study 95 overweight individuals consuming 1.5 oz. of MTNs (n=56) or pretzel snack (n=38) daily as part of a hypocaloric (-500kcal) weight loss diet for 12 weeks followed by an isocaloric weight maintenance program for additional 12 weeks. Trp metabolites kynurenine (KYN), kynurenic acid (KYNA), Trp, indole sulfate (IS), indole propionate (IPA) and indole acetate (IAA) were analyzed by LC-MS and HPLC. Gut microbiota will be analyzed by 16S rRNA sequencing.

Results: At baseline, a positive correlation was observed between heart rate and serum KYN levels and KYN/Trp ratio, as well as between BMI and KYN/Trp ratio. Compared to baseline, KYN levels were significantly reduced in the MTN group at the end of 12 week weight loss phase (P=0.04) and returned to baseline concentration after 12 weeks of isocaloric weight maintenance program (baseline vs. week 12 vs. week 24: 357± 124 vs. 339 + 103 vs. 362 ± 137 ng/mL), but not in pretzel group (418 + 431 vs. 347 ± 150 vs. 375 + 171 ng/mL). Other Trp metabolites evaluatedwere not changed during intervention in either groups. The gut microbiome analysis is ongoing.

Conclusions: We identified a positive association between Try-KYN metabolism and heart rate and BMI in overweight individuals. MTN consumption may directly affect Trp-KYN metabolism.

Funding Sources: International Tree Nut Council Nutrition Research and Education Foundation