(PO25-16-22) Effects of 8 Weeks Feeding Diet With Low and High Isoflavones Soy Protein Concentrate on Serum Isoflavones in Non-alcoholic Fatty Liver Disease (NAFLD) Rat Model

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Presenting Author(s)

RH

Reza Hakkak, PhD
– Professor, UAMS Department of Dietetics and Nutrition, Little Rock, Arkansas, United States

Co-Author(s)

NT

Nathan Twaddle
– National Center for Toxicological Center
BS

Beverly Spray, Ph.D
– Biostatistician, Arkansas Children's Research Institute, Little Rock, Arkansas, United States
DI

David Irby, MS
– Research Assistant, Arkansas Children's Research Institute, Little Rock, Arkansas, United States
SK

Soheila Korourian, MD
– Professor, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States

Disclosure(s):

Reza Hakkak, PhD: No relevant financial relationship(s) with ineligible companies to disclose.

Objectives: To examine the effects of 8 weeks feeding of low and high isoflavones levels in soy protein concentrate on the total serum isoflavones in a NAFLD obese rat model.

Methods: 42 six-week old lean (L, n=21) and obese (O, n=21) Zucker rats were randomly assigned to 1 of 3 dietary groups: casein diet (C=control), soy protein with low isoflavones (LIF), or soy protein with high isoflavones (HIF) for 8 weeks (n=7 rats/group). Rats were weighed twice weekly. After 8 weeks, all rats were sacrificed and serum for total isoflavones (genistein, daidzein and equol) were measured using a validated UPLC-MS/MS method and quantified utilizing stable-labeled internal standards.

Results: Obese rats gained significantly more weight (P < 0.0001) than lean rats for all three diet groups. There was no detectable serum IF for both LC and OB groups. The mean ± SD for LIF vs HIF serum total IF (uM) for genistein were; (0.11+0.04 vs 2.55+1.04; p< 0.001), daidzein (0.07+0.02 vs 1.14+0.45; p< 001) and equol (0.07+0.03 vs 1.84+0.8; p< 0.001). For OLIF vs OHIF serum genistein were; (0.22+0.08 vs 3.69+1.52; p< 0.001), daidzein (0.12+0.04 vs 1.58+0.70; p< 001) and equol (0.06+0.03 vs 2.40+0.7; p< 0.001). Comparing serum genistein LLIF vs OLIF; p< 0.05 for Daidzein p< 0.05 and equol p=0.86.

Conclusions: High isoflavones soy protein concentrate compared to low isoflavones increased serum genistein and daidzein but had no effects on serum equol in a NAFLD rat model.

Funding Sources: This project is support USDA #12889791 to RH.