(PO07-50-22) The Efficacy of Encapsulated Gamma-Cyclodextrin/Tributyrin on Growth and Inflammation in a Dextran Sodium Sulfate-Challenge Piglet Model

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Presenting Author(s)

MM

Marcia H. Monaco, PhD
– Research Assistant Professor, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States

Co-Author(s)

YL

Youngsoo Lee, PhD
– University of Illinois at Urbana-Champaign
Sharon M. Donovan, PhD, RD
– Professor, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States

Disclosure(s):

Marcia H. Monaco, PhD: No relevant financial relationship(s) with ineligible companies to disclose.

Objectives: Inflammatory bowel diseases are common in adults, and their incidence is increasing in children. Butyrate, a short chain fatty acid, can improve gut health, but its bitterness limits incorporation into foods. Herein, the efficacy of tributyrin (TB), a butyrate analog, encapsulated with ɣ-cyclodextrin (ɣ-CD) on growth and intestinal inflammation in a piglet model of dextran-sodium sulfate (DSS)-induced colitis was investigated.

Methods: Two-day-old piglets were randomized to three diets: control (formula alone, CON, N=41), CD (CON + 9.0 mM ɣ-CD, N=41), or CDTB (CON + 9.0 mM ɣ-CD/TB, N=43). Half of the piglets in each group received DSS (1.25g/kg body weight) from postnatal day (PND) 14 to 18. Blood and intestinal samples were collected on PND 19 and 27. IL-6 and TNF-α concentration in serum and colon mucosa were assessed by ELISA and IL-6, TNF-α, IL-10 and IFN-γ gene expression in colonic tissue were measured by qPCR. A quantitative histological grading system was used to assess 4 independent parameters: epithelial lining, cell infiltration, crypt damage and extent of the intestinal section affected.

Results: Average daily weight gain (WG) between PND2-10 and 10-19 were affected by diet (p < 0.05), while colitis led to lower WG from PND19-27. A diet and colitis interaction indicated that after PND22 CDTB-COL piglets weighed less than CON and CD (p=0.051). Diet had no impact on the descending colon (DC) morphology in the colitis animals, but the severity of the DC inflammation increased from PND19 and 27 (p < 0.0001). TNF-α and IL-6 concentrations were elevated in the ascending colon (AC) at PND19, but by PND27 concentrations were similar to non-colitis levels. A similar pattern was found in the DC, except that IL-6 remained elevated on PND27. IL-6, TNF-α and IFN-γ gene expression were significantly higher in the DC of colitis vs. non-colitis animals on PND27. Diet and colitis had no effect on serum TNF-α and IL-6.

Conclusions: The piglet as a model of DSS colitis is a feasible alternative to rodents. Dietary ɣ-CD/TB reduced growth rate and did not ameliorate the inflammatory process of DSS-induced colitis in neonatal pigs. Herein, oral administration of encapsulated TB did not reduce DSS-induced tissue damage or inflammation, however, additional studies are needed to confirm these observations.

Funding Sources: NIFA, ISDA 2017-67017-26519