(PO14-38-22) PGC-1α Is Indispensable in Purple Potato Promoted Intestinal Epithelial Differentiation and Mitochondrial Biogenesis

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Presenting Author(s)

Qi Sun, Master
– PhD candidate, Washington State University, PULLMAN, Washington, United States

Co-Author(s)

AB

Alejandro Bravo Iniguez, PhD student
– Graduate Research Assistant, Washington State University, Pullman, Washington, United States
QT

Qiyu Tian, PhD student
– Washington State University, Pullman, Washington, United States
MD

Min Du, PhD
– Washington State University, Pullman, Washington, United States
MZ

Mei-Jun Zhu, PhD
– Washington State University, Pullman, Washington, United States

Disclosure(s):

Qi Sun, Master: No relevant financial relationship(s) with ineligible companies to disclose.

Objectives: Purple potato (PP) is a good dietary source of polyphenol and promotes gut epithelial health. The objective of this study is to examine the impact of PP extract on mitochondrial biogenesis and evaluate the role of peroxisome proliferator-activated receptor gamma coactivator (PGC)-1α in this.

Methods: In cultured colonic epithelial Caco-2 cells, the effects of PP extract on PGC-1α, cell differentiation, and markers of mitochondrial biogenesis, including mitochondrial DNA content, mitochondrial enzyme gene expression, and levels of metabolic intermediates, were first evaluated. Then, inhibition of PGC-1α induced by either inhibitor SR-18292 or siRNA-mediated knockdown were used to evaluate its role in PP extract-promoted differentiation and mitochondrial biogenesis.

Results: PP extract up-regulated the level of PGC-1α, mitochondrial gene expression, mitochondrial DNA (mtDNA) copy number, and the level of citric acid cycle metabolites in Caco-2 cells. Inhibition of PGC-1α eliminated the enhancing effects of PP extract on the expression of differentiation markers, mitochondrial metabolites and mtDNA content, as well as the mRNA expression of mitochondria-encoded proteins.

Conclusions: PP extract promoted the differentiation of intestinal epithelial cells, mitochondrial biogenesis and oxidative metabolism, which is in a PGC-1α-dependent way.

Funding Sources: United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) (2018-67017-27517) and Washington State University Agricultural Research Center Emerging Research Issues Competitive Grant