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Session: Physiology and Endocrinology I: Metabolism, Endocrine, and/or Stress Interactions

278 - Awardee Talk: Progesterone Regulates the Abundance of mRNAs and Proteins Involved in Polyamine Metabolism in the Sheep Endometrium

Monday, June 27, 2022

8:35 AM – 9:05 AM CT

Location: Oklahoma City Convention Center, 207 A/B

Presenting Author(s)

KH

Katherine M. Halloran

Texas A&M University

Author(s)

EH

Emily Hoskins

Texas A&M University
CS

Claire Stenhouse

Texas A&M University
RM

Robyn Moses

Texas A&M University
MS

Michael C. Satterfield

Texas A&M University
KD

Kathrin Dunlap

Texas A&M University
College Station, TX
HS

Heewon Seo

Department of Veterinary Integrative Biosciences, Texas A&M University
GJ

Gregory A. Johnson

Department of Veterinary Integrative Biosciences, Texas A&M University
College station
Guoyao Wu

Texas A&M University
College Station, Texas
FB

Fuller W. Bazer

Professor
Department of Animal Science, Texas A&M University and Texas A&M AgriLife Research
College Station, TX

Abstract: Agmatine and polyamines (putrescine, spermidine, and spermine), metabolites of L-arginine, are important for development of mammalian conceptuses. The objective of this study was to determine if exogenous progesterone administered during the pre-implantation period, which accelerates conceptus development, alters the expression of mRNAs and proteins involved in the metabolism of agmatine and polyamines in the sheep uterus during pregnancy. Mature Suffolk ewes were bred to fertile rams and assigned randomly to be treated with daily i.m. injections of either 1ml corn oil (CO;n=28) or 25mg progesterone in corn oil (P4;n=20) from Day 1.5 through Day 8 after breeding (Day 0). Ewes were hysterectomized on Day 9, 12, or 125 of gestation and endometrial samples were collected. The expression of candidate mRNAs was quantified using qPCR and proteins localized using immunohistochemistry. On Day 9 of pregnancy, the expression of mRNAs for AMD1 (P < 0.01), ASL (P < 0.01), and SLC12A8 (P < 0.05) was greater in endometria of P4-treated compared to CO-treated ewes. The expression for MAT2B mRNA was greater (P < 0.001) for P4-treated compared to CO-treated ewes on both Days 9 and 12. On Day 125 of gestation, endometria from P4-treated ewes tended to have greater expression of SAT1 (P < 0.10) mRNA compared to CO-treated ewes. In contrast, the expression of AMD1(P < 0.01), MAT2B (P < 0.05), OAT (P < 0.05), SMOX (P < 0.05), and SLC12A8 (P < 0.05) mRNAs was lower in endometria from P4-treated compared to CO-treated ewes on Day 125. Exogenous P4 appeared to increase the relative abundance of SAT1 and SMOX proteins in the uterine luminal and glandular epithelia on Day 125 of pregnancy. These results suggest that early administration of progesterone may alter the biosynthesis of agmatine and polyamines in the sheep uterus during the pre-implantation period of pregnancy with potential ‘programming’ effects manifested in late pregnancy.