162 - Differential Late Gestation Metabolizable Energy Intake Alters Adipose-specific Insulin Responsiveness in Antepartum Beef Cattle

Tuesday, June 28, 2022

5:00 PM – 5:15 PM CT

Location: Oklahoma City Convention Center, 302A

Presenting Author(s)

Koryn S. Hare

Ph.D. Candidate
University of Guelph
Saskatoon, SK

Author(s)

Emily Croft

M.Sc. Student
University of Guelph
Guelph, Ontario
Gregory B. Penner, Ph.D., M.Sc., B.Sc.

Professor
Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
Saskatoon, SK
KW

Katharine M. Wood, n/a

Assistant Professor
University of Guelph
Guelph, ON
MS

Michael A. Steele

Professor
University of Guelph
Guelph, ON

Abstract: We evaluated how metabolizable energy (ME) intake influences antepartum insulin sensitivity in beef cattle. Angus-Simmental cattle (primiparous: n = 17; multiparous: n = 21) were randomly assigned to treatments supplying 80 (LowME, n = 19) or 120% (HighME, n = 19) of predicted ME requirements for 53 d prepartum. At 7 d antepartum, intravenous glucose tolerance tests (IVGTT) were imposed by infusing 1.36 g glucose/kg BW^0.75 and sampling from -3 to 90 min to measure plasma glucose and insulin and serum non-esterified fatty acids (NEFA) concentrations. Data were analyzed using PROC GLIMMIX with the fixed effects of treatment, parity, time (repeated), and treatment×time and considering cow(treatment) as random. Baseline glucose concentration increased (P = 0.03) and baseline insulin concentration tended to increase (P = 0.06) for HighME relative to LowME cattle irrespective (P ≥ 0.33) of parity, whereas baseline NEFA concentration was elevated (P < 0.01) for LowME vs. HighME. Plasma glucose-insulin concentrations during the IVGTT did not differ (P ≥ 0.25) by treatment, treatment×time, or parity. However, serum NEFA was elevated (P < 0.01) throughout the IVGTT for LowME vs. HighME cattle and tended to be affected (P = 0.09) by the treatment×time interaction as NEFA concentrations became similar at 90 min due to HighME cattle having a shallower (treatment×time: P < 0.01) decremental NEFA response than LowME cattle. Low ME cattle cleared NEFA more quickly (75% increase; P < 0.01) than HighME. The glucose positive incremental area-under-the-curve throughout the IVGTT tended to be lesser (P = 0.06) for heifers than cows but heifers had a blunted (P ≤ 0.05) glucose-stimulated reduction in NEFA and NEFA clearance rate compared to cows. These data demonstrate that ME provision may alter adipose insulin responsiveness in antepartum beef cattle and that heifers respond to excess glucose and NEFA differently than cows.