PSVII-16 - Evaluation of the Population Profile of Methanogenic Microorganisms in Animals Treated with the Additive Fator P

Abstract: The effects caused by climatic variations in the world, have been attributed to the increase in the emission of greenhouse gases and as a consequence of worsening global warming. The improper handling of animals can contribute to the emission of gases of enteric origin, which are harmful to the environment. The use of additives in the diet of animals tends to be more advantageous as it will equilibrate the ruminal microbial flora, modulate the ruminal metabolism, and reduce the emission of greenhouse gases to environment. Fator P is a natural additive that was developed to attend the current needs of the livestock market, and make the management system more productive and sustainable, and reduce impacts on the environment. The aim of this work was to evaluate the profile of methanogenic microorganisms by the method of relative quantification by qPCR in animals treated with the natural additive Fator P. The experiment was carried out in triplicate, with a total of six animals, in pasture, receiving 6 gr of the Fator P additive and control. Over 28 days of experiment, the ruminal liquid was collected in days 0, 2, 4, 7, 10, 14, 21, 28, and the DNA was extracted using the Qiagem 51604 kit. The reaction of qPCR was realized using a primers sequence of total bacteria and methanogenic bacteria. The population profile of microorganisms over the days, Factor P showed a statistically significant reduction (p < 0,05) on days 2 (55,7%; p = 0,0188), 7 (72,08%; p = 0,0445), 14 (56,31%, p = 0,0123), 21 (46,62%, p = 0,0049), 28 (53,28%, p = 0,0499). The result indicates that the reduction in the population of methanogens can reduce the emission of gases of enteric origin and shows the efficiency of the additive in management system more productive and sustainable.