NU07 - Evaluation of the IDEXX SediVue Dx® for Identification of Canine and Feline Bacteriuria

Abstract: Background - Detection of bacteriuria is important when evaluating patients with lower urinary tract symptoms. There is a need for accurate and rapid testing to confirm the presence of bacteria in urine samples.

Hypothesis/Objective - The IDEXX SediVue Bacteria Confirmation Kit™ will increase the specificity of SediVue Dx® bacteriuria detection system.

Animals - 122 client-owned dogs and cats presenting to the nephrology/urology service.

Methods - Patients requiring urinalysis and urine culture as part of their diagnostic evaluation were included in the study. Urine samples obtained between Nov 2020 and Nov 2021 analyzed on IDEXX SediVue Dx® and submitted for quantitative urine culture were included. Samples were recorded as positive or negative for bacteria by the SediVue. Samples with “suspect presence” of bacteria were further evaluated using the SediVue bacteria confirmation kit™ as prompted by the SediVue. All urine samples were submitted to an external lab for quantitative urine culture.

Results - 197 urine samples from 103 dogs and 19 cats, of all sexes and breeds were evaluated. Sensitivity of the SediVue analysis for initial detection of bacteria was 85.5%, specificity was 70.2%, positive predictive value (PPV) was 64.4%, and negative predictive value (NPV) was 88.5%. After prompted bacterial confirmation, revised sensitivity/specificity was 81.6% and 90.9% and PPV/NPV was 84.9% and 88.7%.

Conclusions and Clinical Importance – When compared to quantitative urine culture, the addition of the IDEXX bacteria confirmation kit™ to the SediVue Dx® increases both specificity and PPV when determining the presence of bacteria in a urine sample.