ID04 - Evaluation of MiQLab System to Detect Bacterial Pathogens and Antimicrobial Resistance Genes in Bacterial Isolates

Bacterial culture and antimicrobial susceptibility testing is considered the gold standard to confirm the presence of an infection and antimicrobial resistance, but typically requires 3-5 days to obtain results. Genotypic detection of specific pathogens and their associated antimicrobial resistance genes can be used to predict antimicrobial resistance in bacterial pathogens and can deliver accurate results in hours rather than days.
This study was carried out as a proof-of-principle evaluation of MiQLab System to identify bacterial pathogens and their resistance genes. The MiQLab System equipped with MiQLab Bacterial and AMR test V2 can detect 10 common bacterial pathogens infecting cats and dogs and resistance genes for common first-line veterinary antimicrobials such as beta-lactams, sulphonamide-trimethoprim combinations, lincosamides and tetracyclines.
The study evaluated the ability of the MiQLab to detect bacterial pathogens and resistance genes in highly characterised bacterial isolates with available genetic information. The overall percent agreement for bacterial identification between the MiQLab and bacterial culture was 100%. Similarly, the overall percent agreements between antimicrobial resistance gene detection on the MiQLab and genetic sequence data was 96.0%.
These results indicate that MiQLab’s qPCR-based genotypic detection technology can be used to identify bacterial pathogens and their associated antimicrobial resistance genes accurately with a rapid turn-around time.