0683: ANA-positive versus ANA-negative Antiphospholipid Antibody-positive Patients Without Other Systemic Autoimmune Diseases: Clinical and Serological Characteristics, Results from the APS ACTION Clinical Database and Repository
Irene Cecchi1, Massimo Radin2, Silvia Grazietta Foddai2, Maria Laura Bertolaccini3, Danieli De Andrade4, Maria Tektonidou5, Vittorio Pengo6, Guillermo Ruiz-Irastorza7, H. Michael Belmont8, Maria Gerosa9, Paul Fortin10, Chary Lopez-Pedrera11, Zhouli Zhang12, Tatsuya Atsumi13, Guilherme de Jesus14, Hannah Cohen15, Nina Kello16, Ware Branch17, Denis Wahl18, Laura Andreoli19, Esther Rodriguez20, Michelle Petri21, Megan R.W. Barber22, Ricard Cervera23, Jason Knight24, Bahar Artim-Esen25, Rohan Willis26, Guillermo Pons-Estel27, Doruk Erkan28 and Savino Sciascia1, 1University of Turin, Torino, Italy, 2University of Turin, Turin, Italy, 3King's College London, London, United Kingdom, 4University of São Paulo, São Paulo, Brazil, 5University of Athens, Athens, Greece, 6University of Padova, Padova, Italy, 7University of the Basque Country, Barakaldo, Spain, 8New York University School of Medicine, New York, NY, 9University of Milan, Milano, Italy, 10CHU de Québec Université Laval, Québec, QC, Canada, 11Hospital Universitario Reina Sofía, Cordoba, Spain, 12Beijing Medical University, Beijing, China, 13Hokkaido University, Sapporo, Japan, 14Universidade do Estado do Rio de Janeiro, Rio De Janeiro, Brazil, 15University College London, London, United Kingdom, 16Northwell Health, New York, NY, 17University of Utah Health, Salt Lake City, 18Nancy University Hospital, Nancy, France, 19University of Brescia, Brescia, Italy, 20Hospital Universitario 12 de Octubre, Madrid, Spain, 21Johns Hopkins University School of Medicine, Baltimore, MD, 22University of Calgary, Calgary, AB, Canada, 23University of Barcelona, Barcelona, Spain, 24University of Michigan, Ann Arbor, MI, 25Istanbul University, Istanbul, Turkey, 26University of Texas, Galveston, TX, 27Centro Regional de Enfermedades Autoinmunes y Reumáticas, Rosario, Argentina, 28Barbara Volcker Center for Women and Rheumatic Diseases, New York, NY
Background/Purpose: APS ACTION is an international Clinical Database and Repository of persistently antiphospholipid antibody (aPL)-positive subjects, collecting demographic, medical history, and aPL data.This study focused on the prevalence of antinuclear antibodies (ANA) in aPL positive patients without a defined concomitant autoimmune disease. The objective of this study was to evaluate potential differences when stratifying patients by ANA, and to better phenotype aPL positive patients.
Methods: Data from aPL positive patients with or without APS classification criteria were retrieved from the APS ACTION Database. Patients with a diagnosis of systemic lupus erythematosus (SLE) or other connective tissue disease were excluded. Patients were divided in two groups (ANA+ and ANA-), based on ANA status at registry entry. Subsequently, demographic, clinical (including 1997 ACR SLE classification criteria), and serological data were compared between the two subgroups.
Results: 521 aPL-positive patients were included in the study (meanage 52.1±13 years, 70% female). Among them, 224 patients were ANA+ and 297 ANA-. Patients characteristics are displayed in Table 1. ANA positivity was significantly associated with previous history of hematological manifestations as a whole, including hemolytic anemia, thrombocytopenia, and leukopenia, (19.3% ANA+ vs. 8.4% ANA-, p < 0.01) and livedo reticularis (15.1% ANA+ vs. 10% ANA-, p < 0.05). A positive association with the number of unexplained fetal deaths beyond 10 weeks of gestation was also noted (p < 0.05), and a trend was observed for lower platelet count, aPL-related nephropathy and arthritis, though these associations were not statistically significant. No significant association was found for extra-criteria manifestations such as haemolytic anemia and history of thrombocytopenia, when considered individually. When sub-analysing the ANA- group, a significant association with any history of arterial thromboses (29.4% ANA+ vs. 38.8% ANA-, p < 0.02) and the number of arterial events was observed (p < 0.01). When evaluating ANA positivity in aPL carriers and primary APS (PAPS) individually, the association between ANA+ and previous hematologic disorder remained significant for both groups, with stronger significance for PAPS patients. In addition, ANA positivity in PAPS patients was significantly associated with livedo reticularis and previous history of small vessel disease (p < 0.05).
Conclusion: In this large international cohort, ANA positivity was associated with a higher rate of hematologic manifestations in aPL-positive patients without connective tissue disease. ANA+ patients, especially those with PAPS, showed a tendency toward a higher rate of microvascular manifestations and arthritis. ANA- subjects showed a significantly higher rate of arterial thrombosis, without any other significant association with clinical, serological or demographic data. Table 1. Demographic, clinical and serological characteristics of the patients. Disclosures: I. Cecchi, None; M. Radin, None; S. Foddai, None; M. Bertolaccini, None; D. De Andrade, None; M. Tektonidou, None; V. Pengo, None; G. Ruiz-Irastorza, None; H. Belmont, None; M. Gerosa, None; P. Fortin, None; C. Lopez-Pedrera, None; Z. Zhang, None; T. Atsumi, None; G. de Jesus, None; H. Cohen, None; N. Kello, None; W. Branch, None; D. Wahl, None; L. Andreoli, None; E. Rodriguez, None; M. Petri, None; M. Barber, None; R. Cervera, None; J. Knight, None; B. Artim-Esen, None; R. Willis, None; G. Pons-Estel, None; D. Erkan, None; S. Sciascia, None.