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Pulp Therapy
Purpose: The purpose of this study was to evaluate the antibacterial, biocompatible, and mineralization-inducing potentials of different calcium silicate-based pulp capping materials.
Methods: Six contemporary calcium silicate-based pulp capping materials were compared in this study, including ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2 and NeoPutty. The antibacterial effects of these materials against Streptococcus mutans UA159 and Enterococcus faecalis ATCC 29212 were determined by an agar-diffusion assay and a transwell antibacterial assay. The biocompatibility and mineralization-inducing potentials of these materials in preodontoblastic 17IIA11 cells were determined by a MTT assay and by quantification of alkaline phosphatase activity and calcium deposits, respectively.
Results: Antibacterial effects against S. mutans were observed with Biodentine. Minimal antibacterial effects were seen with other tested materials against S. mutans and E. faecalis. All the tested materials except NeoMTA 2 showed favorable effects on preodontoblastic cell proliferation, alkaline phosphatase activity, and formation of mineralized nodules.
Conclusions: Different calcium silicate-based pulp capping materials may have different antibacterial, biocompatible and mineralization-inducing potentials. Understanding the biological properties of pulp capping materials will provide a mechanistic base of their clinical performance on maintaining pulp vitality and will guide the development of new pulp capping materials.