OR15 - Morphological Changes in Human Periodontal Ligament Fibroblasts in Contact with Root Restorative Materials

Abstract: The core function of root restorative materials is to provide root seal, immune modulation, and physiological integration with surrounding tissues. Providing a favorable surface for PDL fibroblast growth and function becomes essential. The direct effect of these restorative materials on PDL fibroblast morphology and behavior hasn’t been well investigated. In this study, human primary periodontal ligament fibroblasts (PDLF) (ATCC) were grown over plates coated with a thin layer of Bioceramic/mineral trioxide aggregate in two forms, NeoMTA and NeoPutty (Avalon Biomed®), Bioceramic (BC) Putty (Brasseler®), ParaCore (Coltene®), or Flowable nanohybrid composite (Henry Shein®). Cells were incubated at 37o C with 5% CO2 in a microscope stage-top incubator (Tokai Hit) and time-lapsed imaging was performed over 48 hours of three randomly selected fields per sample with 150-300 cells/field. Cells morphology and motility were measured by NIS-elements (Nikon) software and data were further analyzed using one-way ANOVA with Bonferroni post hoc test. Only NeoMTA, NeoPutty and BC putty allowed for PDLF growth and proliferation. The average individual cell area was not affected by BC putty; however, it was decreased when growing over NeoMTA and NeoPutty (p< 0.0001). When quantifying the cells’ width and length, no changes were observed with BC putty. Cells became shorter when growing on NeoMTA and Neo Putty (p< 0.0001). Furthermore, cells didn’t show changes in speed, acceleration or distance traveled when growing on any of the materials tested. Conclusion: BC putty maintained human PDL cells’ healthy morphology. However, NeoMTA and NeoPutty caused PDL cell shrinkage and circular formation.