(PR11) Potential Biological Markers Associated with Invasive Cervical Resorption in Gingival Crevicular Fluid: A Split Mouth Pilot Study

Abstract: Invasive cervical resorption (ICR) is an uncommon condition that progresses from the external root leading to compromised tooth structure and ultimately, tooth loss. Pulp, gingival diseases, and other resorptive lesions have been shown to alter the composition of inflammatory markers in the gingival crevicular fluid (GCF). This study, for the first time, seeks to develop a profile of biological mediators found in the GCF of teeth that are directly implicated in ICR. The GCF of twenty-eight teeth affected with ICR and corresponding healthy control teeth in the opposite arch were sampled within the same individual (N= 24). The levels of biological mediators, specifically IL-1RA, IL-1b, TNF-a, OPN, OPG, and RANKL, were analyzed in conjunction with the severity of the ICR condition. Pretreatment demographics and dental history data were collected. Cone-beam computed tomography and periapical images were obtained, and resorptive lesions were classified according to Heithersay classification by two reviewers. Cohen’s kappa coefficient was calculated and regression analyses were performed. Preliminary results reveal the following patient distribution: living with a cat (70%), clenching or grinding history (65%), male (61%), periodontal surgery history (30%), dental trauma history (22%), and bleaching history (17%). Severity of ICR lesions was classified: Heithersay class 4 (30%), 3 (43.3%), 2 (23.3%), and 1 (3.3%). Cohen's kappa coefficient was 0.90. The result of the biological marker analysis is pending laboratory tests. Patient demographics with ICR and stages of disease were analyzed. Laboratory biomarkers results will be available at the time of presentation.