Session: CSAS Graduate Student Oral Competition: MS

150 - Effect of Ergot Alkaloids and a Mycotoxin Deactivating Product on in vitro Rumen Fermentation Using the Rumen Simulation Technique (RUSITEC)

Wednesday, July 14, 2021

1:15 PM – 1:30 PM EST

Location: Kentucky International Convention Center (KICC), M100/M103

Presenting Author(s)

Jenna Sarich, B.Sc.

Masters student
University of Saskatchewan
Craik, Saskatchewan

PDF Slides

Author(s)

KS

Kim Stanford

University of Lethbridge
Lethbridge, Alberta
KS

Karen S. Schwartzkopf-Genswein, n/a

Senior Research Scientist
Agriculture and Agri-Food Canada
Lethbridge, Alberta
Tim A. McAllister, PhD, MSc, BSc

Principal Research Scientist
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, T1J 4B1.
Lethbridge, Alberta
BB

Barry Blakley

University of Saskatchewan
Saskatoon, Saskatchewan
Gregory B. Penner

Associate Professor
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
Saskatoon, Saskatchewan
Gabriel O. Ribeiro, n/a

Assistant Professor
University of Saskatchewan
Saskatoon, Saskatchewan

A rumen simulation technique (RUSITEC) investigated effects of ergot alkaloids (E) and a mycotoxin deactivating product (Biomin® AA; B) on nutrient digestion, rumen fermentation, total gas and methane emissions. Fermenters received a finishing diet of 90:10 concentrate:barley silage. Using a randomized complete block design, treatments were assigned (4 vessels/treatment) within two RUSITECs in a 2 × 2 factorial. Treatments included: 1) control diet, 2) control + 1 g B, 3) control + 20 ppm E, and 4) control + 20 ppm E + 1 g B. The study had a 14-d experimental period, with 7-d adaptation and 7-d sample collection. Data were analyzed in SAS using PROC mixed including fixed effects of E, B, and E×B interaction. Random effects included RUSITEC apparatus and cow rumen inoculum (n = 4). Ergot decreased dry matter disappearance (DMD) (P < 0.05; 87.9 vs. 87.2%) and organic matter disappearance (OMD) (P < 0.05; 88.8 vs. 88.4%). Adding B increased OMD (P < 0.05; 88.3 vs. 88.9%) and neutral detergent fiber disappearance (NDFD) (P < 0.01); however, an E×B interaction was observed for NDFD (P < 0.01) with B promoting greater increases with E. Ergot decreased acetate proportions (P < 0.01) and increased isovalerate (P < 0.05). Consequently, acetate:propionate was reduced (P < 0.05) with E. Inclusion of B increased total volatile fatty acids (P < 0.01), and proportions of acetate (P < 0.05) and propionate (P < 0.05), and decreased valerate (P < 0.01), isovalerate (P < 0.01), and caproate (P < 0.01). Treatments did not affect (P ≥ 0.17) ammonia-N, total gas, or methane production (mg/d or mg/g of OM fermented). In conclusion, E reduced OMD and acetate production, but these responses were reversed by the addition of B.