Session: Ruminant Nutrition IV: Dairy and Fermentation

316 - Determination of the Chemical and Phytochemical Composition of the Methanolic Extract of Urochloa Humidicola for Use as a Phytogenic Additive in Ruminant Diets

Friday, July 16, 2021

2:45 PM – 3:00 PM EST

Location: Kentucky International Convention Center (KICC), L005/009

Presenting Author(s)

Rafaela Scalise Xavier de Freitas, Master

PhD student
Department of Animal Science, College of Animal Science and Food Engineering, Pirassununga, SP, Brazil.
Pirassununga, Sao Paulo

PDF Slides and Audio

Author(s)

TP

Tatiane Pires Pereira

PhD
Federal Rural University of Rio de Janeiro
Viçosa, Minas Gerais
Danny Alexander Rojas Moreno, Sr., studen

Master student
Department of Animal Science, College of Animal Science and Food Engineering, Pirassununga, SP, Brazil.
Pirassununga, Sao Paulo
YP

Yuli Andrea Pena Bermudez, n/a

M.Sc.Animal Science
Department of Animal Science, College of Animal Science and Food Engineering, Pirassununga, SP, Brazil.
Soacha, Cundinamarca
AG

Annelise Aila G. Gomes Lobo, n/a

PhD student
Department of Animal Science, College of Animal Science and Food Engineering, Pirassununga, SP, Brazil.
Pirassununga, Sao Paulo
RZ

Rafaela Zuliani Spalato, n/a

Studen
College of Animal Science and Food Engendering, University of São Paulo, Pirassununga/SP, Brazil
Pirassununga, Sao Paulo
AF

Amanda Thaís Ferreira Regacini

Student
1College of Animal Science and Food Engendering. University of São Paulo.
Pirassununga, Sao Paulo
AP

Ana Luiza Priori Da Silva

Student
College of Animal Science and Food Engendering. University of São Paulo.
Pirassununga, Sao Paulo
ID

Ives Claudio C. Da Silva Bueno, n/a

Proffesor
Department of Animal Science, College of Animal Science and Food Engineering, Pirassununga, SP, Brazil.
Pirassununga, Sao Paulo

Plant extract is an excellent alternative to reduce methane emissions and ameliorate ruminal fermentation due the presence of the secondary metabolites, however, there are many studies with plants used on human’s diet that shows a good result but may occur competition and elevate the costs for the system. Plants consumed by animal have secondary metabolites in quantity and diversity, it can be a good strategy. This work aimed to determine the bromatological composition and characterize the Urochloa humidicola methanol extract such as the presence of secondary metabolites. The samples of the leaves of U. humidicola were collected, dried in the shade with temperature below 40°C, ground in a willey mill in 2 mm particles and subjected to cold maceration with methanol. The solution obtained was filtered by filter paper and concentrated in a rotary evaporator and put to finish the process, drying under airflow. The results of bromatological analyses were subjected to ANOVA by comparing averages by Fisher’s test using the 9.1 Saeg software and phytochemical prospecting testes were characterized as the intensity of the presence of each class of metabolites and represented via the cross-system where:(+++) implies a large presence, (++) implies a considerable presence, (+) implies a small presence and (0) implies a no presence. The dry crude methanolic extract was subjected to analysis of chemical composition and phytochemical prospecting. The extract showed 10.20% crude protein and 35% ether extract and traces of fibrous constituents, because the methanol extraction extracted only soluble constituents. A phytochemical prospecting was positive for the presence of saponin. tannin, alkaloids, catechins, non-protein amino acids and saccharides and was negative for the presence of flavonoids and purines. The U. humidicola methanol extract can be used as a phytogenic additive in the feeding of ruminants.