PSXIV-10 - Ruminal microbiome of cattle changes with the feeding of fat-containing fish supplements

There is insufficient information on the effect of different sources of fat in the diet on the activity and composition of the ruminal microbiome of cattle. The aim of the study was to assess the effect of secondary raw materials - fish oil (1 ml per 100 ml of ruminal fluid; FO) on the microbiome rum of cattle against the background of control (c). The experiment was conducted using the ruminal liquid of the Bulls of the dairy breed (age 12 months, The average live weight of 240-245 kg, diet: hay-70%; grain feed-30%). As a model in vitro used incubator ANKOM Daisy II (USA). Microflora was analyzed with the help of high-performance 16S sequencing of RRNA genes (Illumina). (R≤0.05), which included classes of clostridium (28.6%), bacilli (15.1%), Negativicutes (10.5%), two large families Ruminococcaceae (7.6%) and Lachnospiraceae (16.5%) Bacteroids (39.2%), which included Bacteroidia (38.0%), Preellaceae (25.0%), Prevottell (22.4%). (R≤0.05), Verrucomicrobia (4.5%) (R≤0.05). Clostridia bacteria decreased by 13.8% and Negativicutes by 7.2% compared to C. Fo use showed an increase in bacterial content for bacteroids (57.7%) (R≤0.05) and selected families: Prevotellaceae (32.6%), unclassified Bacteroidales (19.02%), Porrophymonadacee (4.7%) and childbirth: Prevotella (28.8%), Paraprevotella (2.4%). and The Class 5 unit (4.5%) for the FO group showed significant growth. This study was carried out with the financial support of the 0761-2019-0005 project.