PSXVI-11 - Reference intervals of concentrations of essential and toxic elements in the hair of Kalmyk cows bred in the Republic of Sakha (Yakutia) of Russia, calculated by the AES-ICP and MS-ICP methods

Reference intervals of concentrations of chemical elements in biological substrates of animals and humans are the main tool for interpreting the results of laboratory studies. However, establishing the reference intervals, it is necessary to take into account the factor of regional characteristics that consist of a number of elements: climate, terrain, the degree of anthropogenic loads, etc. In this regard, the main purpose of this study was to establish reference intervals for the concentration of chemical elements in the hair of cows of the Kalmyk breed bred in a separate biogeochemical province. The studies were carried out on cows of the Kalmyk breed (n = 126; age 3-5 years) bred in the Republic of Sakha (Yakutia) of Russia. Hair samples were taken with the upper withers of an animal. The elemental composition of the samples was estimated according to 25 chemical elements by ICP-AES and ICP-MS. Reference intervals for the content of chemical elements in animal hair were calculated according to the IUPAC recommendations (Poulsen OM et al., 1997) (2.5-97.5 percentile). As a result of the research, the following values of the boundaries of intervals have been established: Al (139-704); As (0.051-0.287); B (0.921-4.95); Ca (1272-3391); Cd (0.002-0.014); Co (0.111-0.517); Cr (0.674-3.09); Cu (4,840-8.99); Fe (250-1120); Hg (0.004-0.052); I (0.266-1.61); K (1433-6687); Li (0.248-0.857); Mg (393-2252); Mn (7.22-65.89); Na (1013-5258); Ni (0.448-2.88); P (160.0-374); Pb (0.170-0.615); Se (0.147-0.419); Si (6.36-604); Sn (0.011-0.630); Sr (4.5-17.80); V (0.401-1.59); Zn (72.93-126.0). The reference intervals obtained in our study can be used to assess the elemental status of the Kalmyk cows bred in the Republic of Sakha (Yakutia) of Russia. The research was carried out in accordance with the state contract No. 19-28 "Implementation of adaptive technologies for keeping specialized beef cattle in the conditions of the Republic of Sakha (Yakutia)."