Session: Late-Breaking Swine Species

512 - Late-Breaking: A Postbiotic from Lactobacillus Acidophilus Fermentation (LAF) Accelerates Progression of Compositional and Functional Gut Microbiome Clusters and Promotes Body Weight in Nursery Pigs

Saturday, July 17, 2021

10:30 AM – 10:45 AM EST

Location: Kentucky International Convention Center (KICC), M101 & 102

Presenting Author(s)

EK

Ehsan Khafipour

Director, Microbiome Research and Technical Support
Diamond V, Cargill Health Technologies
Cedar Rapids, Iowa

PDF Slides and Audio

Author(s)

AC

Anirikh Chakrabarti

Cargill R&D Centre Europe
Vilvoorde, Brabant Wallon
MS

Maria Sardi

Cargill Biotechnology R&D
Minneapolis, Minnesota
BK

Briana Kozlowicz

Cargill Health Technologies
Minneapolis, Minnesota
DP

Derek B. Petry

Diamond V, Cargill Health Technologies
Cedar Rapids, Iowa
AK

Anantawat Koontanatechanon

Feed Technology Department, Charoen Pokphand Group
Bangkok, Buriram
Wp

Wanpuech parnsen

Feed Technology Department, Charoen Pokphand Group
Bangkok, Buriram
PS

Pairat Srichana

Feed Technology Department, Charoen Pokphand Group
Bangkok, Buriram

This study investigated the effects of a postbiotic from Lactobacillus acidophilus fermentation (LAF) on pig gut microbiome during nursery. Piglets (n = 32) at weaning (day 21 ± 2) were randomized based on BW (7.4 ± 1.7 kg) and received basal diets that met NRC nutrient requirements for phase 1 (d 0-14) and phase 2 (d 14-32) post-wean. Treatments included i) NC, negative control; ii) PC, antibiotic positive control, iii) LAFa (Dia-V™ Nursery, Diamond V, IA) supplemented at 1000 ppm in phase 1 and 1500 ppm in phase 2, and iv) LAFb (Dia-V™ Nursery) supplemented at 2000 and 1000 ppm during phase 1 and 2, respectively. Fecal samples were collected on d 0, 7, 14, and 32 post-wean and subjected to DNA extraction and long read Nanopore shotgun metagenomics to assess composition, function and progression of gut microbiome and their correlations with BW. On d7, PC and LAF supplemented pigs numerically were closer to microbiome of d 14 and 32 compared to NC pigs. From d 0 to 32, pigs progressed through five compositional clusters (P < 0.05). By d 32, more pigs from PC and LAF groups were exhibiting a compositional cluster that was characterized by dominance of members of Bacteroidetes phylum including several Prevotella species. From d 0 to d 32, four microbiome functional clusters were observed (P < 0.05) with varying abundances of carbohydrate-active-enzymes (CAZy). Pigs in LAF groups compared to other treatments exhibited clusters with greater abundances of CAZy that was correlated with greater BW on d 32 (P = 0.004). The 2000 ppm supplementation of LAF in phase 1 and 1500 ppm in phase 2 numerically increased all measured of diversity compared to 1000 ppm. Overall, while PC and LAF groups promoted more similar microbiome compositional clusters compared to NC, LAF pigs exhibited superior functional clusters.