Session: Animal Breeding and Genetics V: Genomic Selection II

501 - Late-Breaking: Search for Genomic Associations with Cryo-resistance of Sperm in Animals

Saturday, July 17, 2021

2:30 PM – 2:45 PM EST

Location: Kentucky International Convention Center (KICC), M105 & 106

Presenting Author(s)

Elena Nikitkina

Senior researcher
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Petersburg, Saint Petersburg City

PDF Slides and Audio

Author(s)

ND

Natalia Dementieva

Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Petersburg, Saint Petersburg City
YS

Yuyriy Shcherbakov

PhD
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St. Petersburg, Saint Petersburg City
Anna Krutikova, PhD

senior researcher
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Petersburg, Saint Petersburg City
Artem Musidray, PhD

Junior Researcher
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Petersburg, Saint Petersburg City
NP

Nikolay Pleshanov

Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Petersburg, Saint Petersburg City
OS

Oleg Samoylov

Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
St.Peterburg, Saint Petersburg City

Genetic resistance to sperm cryopreservation is an important factor in the sires selection. In our study, we studied the cryoresistance of the sperm in Gallus gallus, Bos taurus, Equus ferus caballus. Sperm quality was assessed using computer-assisted semen analysis (CASA). Genetic studies were performed using Affymetrix Axiom®600k Array chips for cocks, Affymetrix Axiom®600k Array for stallions and BovineSNP50 for bulls. For the analysis of genotypes, the GWAS (Genome-Wide Association Studies) was used. The calculations were carried out using the PLINK and EMMAX programs. The candidate genes found as a result of our work were compared with the functions of orthologous genes, according to the publications of other researchers. As a result of comparing different animal species, a common candidate gene was found, localized on chromosome 3 (Gallus gallus) and on BTA9 (Bos taurus) BCKDHB gene encodes the beta subunit E1 of branched chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. In roosters, it was associated with the preservation of sperm motility after cryopreservation, and it was found in the analysis of the integrity of the sperm head in bulls. The functioning of genes associated with meiosis and sperm maturation is an important condition for maintaining its quality after freezing. The work of genes responsible for the formation of membranes and various cellular structures associated with the functions of spermatozoa is also an important factor in cryoresistance. Another important condition for the functioning of the sperm is the expression of genes that prepare the sperm for fertilization. These are calcium metabolism genes involved in preparation for capacitation. Changes in the structure of proteins can lead to premature capacitation and disruption of sperm integrity. The found molecular genetic markers will be used to select sires whose sperm are suitable for freezing. Project of RSF № 18-16-00071.