Session: Ruminant Nutrition III: Feedlot Nutrition

312 - Effects of Bismuth Subsalicylate and Different Sulfate Concentrations on in vitro Ruminal Fermentation Using a Warm-season Perennial Hay Substrate

Friday, July 16, 2021

10:30 AM – 10:45 AM EST

Location: Kentucky International Convention Center (KICC), L005/009

Presenting Author(s)

Kenneth S. Madrid

Undergraduate Student
Texas Tech University
Lubbock, Texas

PDF Slides and Audio

Author(s)

Andrea M. Osorio Doblado, MA

Research Assistant
Texas Tech University
Lubbock, Texas
FC

Francine M. Ciriaco, n/a

Post Research Associate
Texas Tech University
Lubbock, Texas
Kymberly D. Coello

Research Scholar
Texas Tech University
Lubbock, Texas
Angel A. Raudales Quijada

Research Scholar
Texas Tech Univeristy
Lubbock, Texas
JO

Jhones Onorino Sarturi, n/a

Associate Professor
Texas Tech University
Lubbock, Texas
Gregory B. Penner

Associate Professor
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
Saskatoon, Saskatchewan
DH

Darren D. Henry, n/a

Assistant Professor
Texas Tech University
Lubbock, Texas

A randomized complete block design was used to evaluate the effects of bismuth subsalicylate (BSS) on in vitro ruminal fermentation with differing concentrations of sulfate. In vitro fermentation consisted of 50 mL of a 4:1 buffer:ruminal fluid inoculum and 0.7 g (pre-dehydrated) of substrate [WW-B Dahl bluestem hay (Bothriochloa bladhii)] incubated for 48 h (39^oC). Treatments were arranged as a 3 × 4 factorial with concentration of sulfate (0.2, 2.9, or 5.6 g sulfate/L buffer) and BSS (0.0, 0.165, 0.330, or 0.495% substrate DM) as the main factors. In vitro organic matter digestibility (IVOMD), and CH₄, H₂S, and total gas production (TGP) were measured. Data were analyzed using the MIXED procedure of SAS with the fixed effects of BSS, sulfate, and their interaction. Incubation day (block) was considered a random effect. The average of 2 bottles within day was considered experimental unit. A BSS × sulfate interaction was observed for TGP (P = 0.040) and H₂S production (P < 0.001), where BSS had a larger negative impact on TGP and production of H₂S with greater concentrations of sulfate. A linear effect (P < 0.001) of sulfate was observed for CH₄ production per gram of incubated OM, where CH₄ was decreased as sulfate concentration increased. A quadratic effect of sulfate was observed for IVOMD (P = 0.010) and pH (P = 0.009). Production of H₂S linearly decreased (P = 0.001) as BSS concentration increased. The addition of BSS to in vitro incubations did not affect (P > 0.10) any other variables measured. Bismuth subsalicylate does not appear to have negative effects on in vitro fermentation parameters while decreasing H₂S production; however, elevated concentrations of sulfate in the buffer appears to have negative impacts on fermentation. Further in vivo research is warranted to support BSS supplementation to cattle with high dietary sulfate.