P0139 - Bioinformatic Analysis of Diagnostic Value of SFRP Family Members in Colon Cancer

Chenyu Sun, MD, MSc¹, John Pocholo W. Tuason, MD¹, Zhichun Guo, ², Na Hyun Kim, MD¹, Nikitha Manem, MD¹, Chandur Bhan, MD¹, Rui Gong, ², Mengyu Chen, ², Danye Zhou, ², Chenyu Cao, ², Zhilin Zhou, ², Apurwa Prasad, MD^1
1AMITA Health Saint Joseph Hospital, Chicago, IL; ²Massachusetts College of Pharmacy and Health Sciences, Boston, MA

Introduction: Colon cancer (CC) is a common malignant tumor with high mortality. The aberrant expression of secreted frizzled-related proteins (SFRPs) was seen in different types of cancer. Therefore, this study was conducted to explore their diagnostic values in CC.

Methods: The expression levels of SFRPs family members between CC and normal tissue were first compared by using Wilcoxon rank-sum test, and visualized by ‘ggplot2’ package of R software version v3.6.3 (The R Foundation for Statistical Computing, 2020). p < 0.05 was considered as statistically significant. Data were extracted from The Cancer Genome Atlas (TCGA) database (https://portal.gdc.cancer.gov/), and Log2 transformed FPKM (fragments per kilobase exon-model per million reads mapped) were used. The best discriminate cut-off point between the high and low expression groups was assessed by the ROC curve and area under the curve (AUC) values for selected hub genes, based on data from The Cancer Genome Atlas (TCGA). R software with pROC and ggplot2 packages was used.

Results: The expression level of SFRP4 was significantly elevated in CC tissues, while SFRP1, SFRP2, SFRP3/FRZB, and SFRP5 were significantly lower in CC tissues than in normal tissues. AUC of SFRP1, SFRP2, SFRP3/FRZB, SFRP4, and SFRP5 were 0.982, 0.785, 0.819, 0.805, and 0.9395, respectively.

Discussion: The expression level of SFRP4 was significantly elevated, while that of other members of the SFRPs family were significantly lower in CC tissues. For CC patients, lower expression of SFRP1 and SFRP5 may provide potential diagnostic values.


Figure: A) Expression level of SFRPs family members in colon cancer tissues versus normal colon tissues; B) ROC curves of SFRPs family members in colon cancer

	Types of EC VS. normal	Fold Change	P-value	t-test	References
SFRP1	Esophageal Adenocarcinoma	3.947	2.10E-4	4.370	Hao Esophagus
SFRP3	Esophageal Adenocarcinoma	3.305	4.10E-16	9.623	Kim Esophagus
	Esophageal Adenocarcinoma	8.689	1.96E-4	4.517	Hao Esophagus
	Esophageal Adenocarcinoma	3.599	0.046	1.874	Kimchi Esophagus
SFRP4	Esophageal Squamous Cell Carcinoma	2.699	1.10E-5	5.674	Hu Esophagus
	Esophageal Squamous Cell Carcinoma	2.312	3.99E-10	7.021	Su Esophagus 2

Table: Table 1. The significant changes of SFRPs expression in transcription level between colon cancer and normal tissues (Oncomine)


Disclosures:
Chenyu Sun indicated no relevant financial relationships.
John Pocholo Tuason indicated no relevant financial relationships.
Zhichun Guo indicated no relevant financial relationships.
Na Hyun Kim indicated no relevant financial relationships.
Nikitha Manem indicated no relevant financial relationships.
Chandur Bhan indicated no relevant financial relationships.
Rui Gong indicated no relevant financial relationships.
Mengyu Chen indicated no relevant financial relationships.
Danye Zhou indicated no relevant financial relationships.
Chenyu Cao indicated no relevant financial relationships.
Zhilin Zhou indicated no relevant financial relationships.
Apurwa Prasad indicated no relevant financial relationships.


Chenyu Sun, MD, MSc¹, John Pocholo W. Tuason, MD¹, Zhichun Guo, ², Na Hyun Kim, MD¹, Nikitha Manem, MD¹, Chandur Bhan, MD¹, Rui Gong, ², Mengyu Chen, ², Danye Zhou, ², Chenyu Cao, ², Zhilin Zhou, ², Apurwa Prasad, MD¹. P0139 - Bioinformatic Analysis of Diagnostic Value of SFRP Family Members in Colon Cancer, ACG 2021 Annual Scientific Meeting Abstracts. Las Vegas, Nevada: American College of Gastroenterology.