P1175 - Effect of Probiotics Mixture Against Clostridium difficile Growth Inhibition

Dong Ho Lee, MD, PhD¹, Jung Hyun Kim, PhD², Ki Sung Kang, ², Jin-Sol Lee, MS², Na Ri Lim, ², Won Suk Lee, ², Young Soo Park, MD¹, Cheol Min Shin, MD¹, Hyuk Yoon, MD¹, Na Young Kim, MD¹, Eun Ji Lee, MD¹, Yu Jin Kim, MD¹, Mun Jin Ju, MD¹, Ye Ji Choi, MD^1
1Seoul National University Bundang Hospital, Seongnam, Kyonggi-do, Republic of Korea; ²BioBankHealing, Seongnam, Kyonggi-do, Republic of Korea

Introduction: Clostridium difficile is the main cause of gastrointestinal illness, ranging from mild to profuse diarrhea with abdominal pain. Many studies have demonstrated that Lactobacillus and Bifidobacterium are associated with colonization resistance against C. difficile. In this study, we investigated whether the combination of Bifidobacterium and Lactobacillus could suppress the growth of C. difficile.

Methods: We selected five strains of lactic acid bacteria (LAB), namely, Bifidobacterium longum, B. adolescentis, Lactobacillus rhamnosus, L. plantarum, L. paracasei subsp. Tolerans isolated from healthy human. Each strain is routinely cultured and collected. To co-culture with C. difficile, the pellet was washed twice in PBS, then inoculated to RCM medium with C. difficile, respectively. Also, the multi-strain combination prepared equal proportion (1:1:1:1:1). Cultures were incubated for 24 hours under anaerobic conditions at 37℃. The antimicrobial ability of multi-strain against C. difficile was determined by evaluating C. difficile growth.

Results: We screened the single-strain and multi-strain combination for the ability to suppress C. difficile growth. Bifidobacterium strain could not inhibition of C. difficile. On the other hand, Lactobacillus strain significantly inhibition of C. difficile compared with Bifidobacterium strain. The combination probiotic mixtures completely could suppress the growth of C. difficile.

Discussion: In this study, we found that multi-strain probiotics were completely able to inhibit the growth of C. difficile. 5 probiotics mixtures showed the inhibition of C. difficile. Probiotics used to potentially prevent or treat CDI function through multiple possible mechanisms. Therefore, These results suggesting that the potential for use in the treatment of Clostridium difficile infection.


Disclosures:
Dong Ho Lee indicated no relevant financial relationships.
Jung Hyun Kim indicated no relevant financial relationships.
Ki Sung Kang indicated no relevant financial relationships.
Jin-Sol Lee indicated no relevant financial relationships.
Na Ri Lim indicated no relevant financial relationships.
Won Suk Lee indicated no relevant financial relationships.
Young Soo Park indicated no relevant financial relationships.
Cheol Min Shin indicated no relevant financial relationships.
Hyuk Yoon indicated no relevant financial relationships.
Na Young Kim indicated no relevant financial relationships.
Eun Ji Lee indicated no relevant financial relationships.
Yu Jin Kim indicated no relevant financial relationships.
Mun Jin Ju indicated no relevant financial relationships.
Ye Ji Choi indicated no relevant financial relationships.


Dong Ho Lee, MD, PhD¹, Jung Hyun Kim, PhD², Ki Sung Kang, ², Jin-Sol Lee, MS², Na Ri Lim, ², Won Suk Lee, ², Young Soo Park, MD¹, Cheol Min Shin, MD¹, Hyuk Yoon, MD¹, Na Young Kim, MD¹, Eun Ji Lee, MD¹, Yu Jin Kim, MD¹, Mun Jin Ju, MD¹, Ye Ji Choi, MD¹. P1175 - Effect of Probiotics Mixture Against Clostridium difficile Growth Inhibition, ACG 2021 Annual Scientific Meeting Abstracts. Las Vegas, Nevada: American College of Gastroenterology.