975 Views
Category: Diagnostics: Bacteriology/mycobacteriology
Poster Session: Diagnostics: Bacteriology/mycobacteriology
Joshua Wolf
Associate member
St. Jude Children's Research Hospital
Memphis, Tennessee
Disclosure: Karius inc (Grant/Research Support)
Kathryn Goggin
Infectious diseases fellow
St. Jude’s Children’s Research Hospital
Memphis, Tennessee
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Amanda griffen
Laboratory technician
St. Jude Children's Research Hospital
Memphis, Tennessee
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Christina Kohler
Laboratory technician
St. Jude Children's Research Hospital
Memphis, Tennessee
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Kim J. Allison
Manager Clinical Research Operations
St. Jude Children's Research Hospital
Memphis, TN
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Yuki Inaba
Medical Student
UTSW
Dallas, Texas
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Desiree D. Hollemon
Head of Clinical Affairs
Karius, Inc
Redwood City, CA
Disclosure: Karius inc (Employee)
Abigail Brenner
Medical Student
Indiana University
Indianapolis, Indiana
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Gabriela Maron
Associate Member
St. Jude Children's Research Hospital
Memphis, TN
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Yilun Sun
Statistician
St. Jude Children's Research Hospital
Memphis, Tennessee
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Li Tang
Associate Member
St. Jude Children's Research Hospital
Memphis, TN
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
Charles Gawad
Assistant Professor
Stanford University
Stanford, California
Disclosure: Karius inc (Grant/Research Support)
Ellie Margolis
Assistant Member
St. Jude Children's Research Hospital
Memphis, Tennessee
Disclosure: I do not have any relevant financial / non-financial relationships with any proprietary interests.
There were 13 evaluable BSI episodes in 9 participants; 7 had slow DNA decay. Persistence of bacteremia or fever ≥1 day after initiation of effective antibiotics occurred in 9/13 episodes (7/7 slow decay and 2/6 rapid decay; P = 0.02). Slow decay persisted beyond resolution of bacteremia and fever in 3/7 of these cases.Figure 1. Pathogen DNA Concentration by mcfDNA-seq During Antibiotic Treatment of Bacteremia; Dashed line, blood culture positive; Red circle, last fever
Conclusion: In this small convenience sample of patients with leukemia, slow mcfDNA-seq DNA decay correlated with persistent fever or bacteremia. Post-BSI mcfDNA-seq monitoring should be investigated with the goal of decreasing inappropriate antibiotic therapy and preventing treatment failure.